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Fluorescent and Colorimetric Ionic Probe Based on Fluorescein for the Rapid and On-Site Detection of Paraquat in Vegetables and the Environment
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-11-23 , DOI: 10.1021/acs.jafc.2c05980 Siying Che 1 , Xiutan Peng 1 , Yiwan Zhuge 1 , Xinlan Chen 1 , ChunSong Zhou 1 , Haiyan Fu 2 , Yuanbin She 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-11-23 , DOI: 10.1021/acs.jafc.2c05980 Siying Che 1 , Xiutan Peng 1 , Yiwan Zhuge 1 , Xinlan Chen 1 , ChunSong Zhou 1 , Haiyan Fu 2 , Yuanbin She 1
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Detection of pesticide paraquat (PQ) is of considerable significance to ensure food safety, and its rapid and on-site detection is still a challenge. Aimed at the ion characteristics of PQ, an “enrichment and detection” strategy was proposed to improve the sensitivity through electrostatic attractions, and the ion characteristic of probes was adopted to increase the portability through avoiding aggregation-caused quenching effects in the paper strips. Herein, a novel anion-functionalized ionic liquid (IL) probe with a large conjugated plane and rich π-electrons ([Fluo][P66614]2) was designed as a fluorescent and colorimetric dual-channel probe to sensitively and rapidly detect trace amounts of PQ in vegetables and the environment. The proposed probe exhibited good linearity with a detection limit of 64.0 nM in the PQ concentration range of 0.3–7.0 μM (fluorometry) and 0.1 μM in that of 0.1–8.0 μM (colorimetry), respectively. In addition, it displayed a rapid fluorescence quenching response from green to dark (<5 s) and excellent anti-interference (among 23 other pesticides) due to dual effects of electrostatic attraction and π–π stacking. Most importantly, the lipophilic IL probe could be applied in real vegetables and environmental samples with a satisfying recovery rate of 98–103% and assembled into a handy paper strip that achieved the visual semiquantitative detection of PQ. This ionic probe provides a feasible approach for rapidly and conveniently detecting PQ for ensuring agricultural and food safety and opens a new avenue to detect ion-responsive analytes in real complex samples by an “enrichment and detection” strategy.
中文翻译：
基于荧光素的荧光比色离子探针用于蔬菜和环境中百草枯的快速现场检测
农药百草枯（PQ）的检测对保障食品安全具有重要意义，其快速、现场检测仍然是一个挑战。针对PQ的离子特性，提出了一种“富集和检测”策略，通过静电吸引提高灵敏度，并利用探针的离子特性，通过避免纸条中聚集引起的猝灭效应来增加便携性。在此，一种具有大共轭平面和丰富 π 电子的新型阴离子功能化离子液体 (IL) 探针 ([Fluo][P 66614 ] 2) 被设计为荧光和比色双通道探针，可灵敏、快速地检测蔬菜和环境中的痕量 PQ。所提出的探针在 PQ 浓度范围为 0.3-7.0 μM（荧光法）和 0.1-8.0 μM（比色法）的 PQ 浓度范围内表现出良好的线性，检测限分别为 64.0 nM 和 0.1 μM（比色法）。此外，由于静电吸引和 π-π 堆积的双重作用，它显示出从绿色到深色（<5 s）的快速荧光猝灭响应和出色的抗干扰性（在其他 23 种农药中）。最重要的是，亲脂性 IL 探针可以应用于真正的蔬菜和环境样品，回收率达到 98-103%，并组装成方便的纸条，实现 PQ 的视觉半定量检测。
更新日期：2022-11-23
中文翻译：
基于荧光素的荧光比色离子探针用于蔬菜和环境中百草枯的快速现场检测
农药百草枯（PQ）的检测对保障食品安全具有重要意义，其快速、现场检测仍然是一个挑战。针对PQ的离子特性，提出了一种“富集和检测”策略，通过静电吸引提高灵敏度，并利用探针的离子特性，通过避免纸条中聚集引起的猝灭效应来增加便携性。在此，一种具有大共轭平面和丰富 π 电子的新型阴离子功能化离子液体 (IL) 探针 ([Fluo][P 66614 ] 2) 被设计为荧光和比色双通道探针，可灵敏、快速地检测蔬菜和环境中的痕量 PQ。所提出的探针在 PQ 浓度范围为 0.3-7.0 μM（荧光法）和 0.1-8.0 μM（比色法）的 PQ 浓度范围内表现出良好的线性，检测限分别为 64.0 nM 和 0.1 μM（比色法）。此外，由于静电吸引和 π-π 堆积的双重作用，它显示出从绿色到深色（<5 s）的快速荧光猝灭响应和出色的抗干扰性（在其他 23 种农药中）。最重要的是，亲脂性 IL 探针可以应用于真正的蔬菜和环境样品，回收率达到 98-103%，并组装成方便的纸条，实现 PQ 的视觉半定量检测。
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