In this study, exogenous Megasphaera elsdenii inoculum and acetate supplementation were introduced at the acidogenic phase to regulate the acidogenic fermentation pathway and assess their effects on food waste (FW) carbon conversion in two-phase anaerobic digestion (AD) system. These two additives significantly accelerated organic removal efficiency and subsequently increased FW hydrolysis and acidogenesis by 16% and 35%, respectively. As expected, two exogenous additives promoted butyrate fermentation during FW acidogenesis. With regard to the role of exogenous additives, both hydrogen and butyrate yields increased by over 60%. This desired increment resulted in a 25% increase in methane production. The overall carbon conversion from FW in the integrated two-phase AD system was enhanced by biochemical additives, which was 1.3-fold higher than that in control without any additives. Collectively, findings demonstrate the feasibility of regulating acidogenic fermentation via exogenous biochemical additives and its benefits on FW carbon conversion during AD process.

在本研究中，外源埃氏巨球藻在产酸阶段引入接种物和乙酸盐补充剂以调节产酸发酵途径，并评估它们对两相厌氧消化 (AD) 系统中食物垃圾 (FW) 碳转化的影响。这两种添加剂显着提高了有机物去除效率，随后分别将 FW 水解和产酸增加了 16% 和 35%。正如预期的那样，两种外源添加剂在 FW 产酸过程中促进了丁酸发酵。在外源添加剂的作用下，氢气和丁酸产率均提高了60%以上。这一理想的增量导致甲烷产量增加 25%。生化添加剂增强了集成两相 AD 系统中 FW 的整体碳转化率，比没有任何添加剂的对照高 1.3 倍。