Urinary proteomics was used to investigate the potential effects of e-cigarettes on the human body. In this study, a rat e-cigarette model was constructed by smoking for two weeks and urine samples before, during, and after e-cigarette smoking were collected. Urine proteomes before-after smoking of each rat were compared individually, while the control group was set up to rule out differences caused by rat growth and development. After smoking, the differential proteins produced by rats shows strong individual variation. Fetuin-B, a biomarker of COPD, and annexin A2, which is recognized as a multiple tumor marker, were identified as the differential proteins in five out of six smoking rats on day 3. To our surprise, odorant-binding proteins expressed in the olfactory epithelium were also found and were significantly upregulated, which may help explain olfactory adaptation. Pathways enriched by the differential proteins shows the evidence that smoking e-cigarettes affects the immune system, cardiovascular system, respiratory system, etc., which provides clues for further exploration of the mechanism of e-cigarettes on the human body.

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大鼠电子烟模型的尿液蛋白质组学分析

尿液蛋白质组学用于研究电子烟对人体的潜在影响。在这项研究中，通过吸烟两周构建大鼠电子烟模型，并收集电子烟吸烟前、吸烟期间和吸烟后的尿液样本。分别比较每只大鼠吸烟前后的尿液蛋白质组，同时设置对照组以排除大鼠生长发育引起的差异。吸烟后，大鼠产生的差异蛋白表现出强烈的个体差异。COPD 的生物标志物 Fetuin-B 和被认为是多种肿瘤标志物的膜联蛋白 A2 在第 3 天被鉴定为六只吸烟大鼠中的五只的差异蛋白。令我们惊讶的是，气味结合蛋白在嗅觉上皮细胞也被发现并显着上调，这可能有助于解释嗅觉适应。差异蛋白富集的通路显示吸食电子烟影响免疫系统、心血管系统、呼吸系统等的证据，为进一步探索电子烟对人体的作用机制提供线索。