Urine proteomics was applied to explore whether the effect of startle can be detected in urine. A combination of natural enemy odor and sound stimulation was used to establish the rat startle model. Urine samples were collected before and after startle, and urine proteomes before-after startle of each rat were compared individually. Regulatory subunits of glutamate-cysteine ligase was iden-tified as the sole differential protein among all five startled rats. To our surprise, its functional partner catalytic subunits of glutamate-cysteine ligase was also identified in four out of five rats as differential protein. When comparing before-after startle as two groups, 22 differential proteins were identified which represent biological pathways including neurotransmitter transport and glucose transmembrane transport.

中文翻译：

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大鼠惊吓模型的尿液蛋白质组学分析

应用尿液蛋白质组学来探索是否可以在尿液中检测到惊吓效应。结合天敌气味和声音刺激建立大鼠惊吓模型。在惊吓前后收集尿液样本，并分别比较每只大鼠惊吓前后的尿液蛋白质组。谷氨酸-半胱氨酸连接酶的调节亚基被确定为所有五只受惊大鼠中唯一的差异蛋白。令我们惊讶的是，它的谷氨酸-半胱氨酸连接酶的功能伙伴催化亚基也在五分之四的大鼠中被鉴定为差异蛋白。当比较两组的惊吓前后时，确定了 22 种差异蛋白，它们代表了包括神经递质转运和葡萄糖跨膜转运在内的生物途径。