Furan fatty acids (FuFA) are important antioxidants found in low concentrations in many types of food. In addition to conventional FuFA which normally feature saturated carboxyalkyl and alkyl chains, a few previous studies indicated the FuFA co-occurrence of low shares of unsaturated furan fatty acids (uFuFA). For their detailed analysis, the potential uFuFA were enriched by centrifugal partition chromatography (CPC) or countercurrent chromatography (CCC) followed by silver ion chromatography from a 4,7,10,13,16,19-docosahexaenoic acid ethyl ester oil, a 5,8,11,14,17-eicosapentaenoic acid ethyl ester oil and a latex glove extract. Subsequent gas chromatography with mass spectrometry (GC/MS) analysis enabled the detection of 16 individual uFuFA isomers with a double bond in conjugation with the central furan moiety. In either case, four instead of two uFuFA isomers previously reported in food, respectively, were detected by GC/MS. These isomers showed characteristic elution and abundance patterns in GC/MS chromatograms which indicated the presence of two pairs of cis/trans-isomers (geometrical isomers).

中文翻译：

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食品中不饱和呋喃脂肪酸的几何和位置异构体

呋喃脂肪酸 (FuFA) 是重要的抗氧化剂，在许多类型的食物中都以低浓度存在。除了通常具有饱和羧基烷基和烷基链的传统 FuFA 之外，之前的一些研究表明 FuFA 与低份额的不饱和呋喃脂肪酸 (uFuFA) 共存。为了进行详细分析，潜在的 uFuFA 通过离心分配色谱法 (CPC) 或逆流色谱法 (CCC)，然后通过银离子色谱法从 4,7,10,13,16,19-二十二碳六烯酸乙酯油、5 ,8,11,14,17-二十碳五烯酸乙酯油和乳胶手套提取物。随后的气相色谱与质谱 (GC/MS) 分析能够检测到 16 种单独的 uFuFA 异构体，这些异构体具有与中心呋喃部分共轭的双键。在任一情况下，GC/MS 分别检测到四种而不是之前在食品中报道的两种 uFuFA 异构体。这些异构体在 GC/MS 色谱图中显示出特征性的洗脱和丰度模式，表明存在两对顺式/反式异构体（几何异构体）。