Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor–insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s.

中文翻译：

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用于连续细胞富集和分离的低成本、高通量和快速原型 3D 集成介电泳通道

用于介电泳细胞分离的微流体装置通常是在洁净室中使用微加工方法设计和构建的，需要时间和费用。在本文中，我们描述了一种微流体设备制造的新颖替代方法，使用刻字机从导体-绝缘体层压板上切割芯片。这使得能够制造沿每个壁具有微米级电极的微通道装置。制造使用传统的桌面刻字机，不需要化学品、面罩或洁净室通道；功能性流体装置可以在几个小时内以可忽略不计的成本设计和构建。作为一个例子，我们展示了连续介电泳细胞分离器的构建，能够以 10 000 个细胞/秒的速度将酵母细胞富集至 80% 的纯度。