In this study, an innovative approach was developed to enhance the hydrolysis through phase-separated pretreatment by removing exopolymeric substances via mild sonication followed by thermo-Fenton disintegration. The exopolymeric substances fragmentation was enhanced at the sonic specific energy input of 2.58 kJ/kg total solids. After exopolymeric substance removal, the disintegration of biomass by thermo-Fenton yield the solubilization of 29.8 % at Fe²⁺:H₂O₂ dosage and temperature of 0.009:0.036 g/g suspended solids and 80 °C as compared to thermo-Fenton alone disintegration. The polyhydroxybutyrate content of 93.1 % was accumulated by Bacillus aryabhattai at the optimum time of 42 h, while providing 70 % (v/v) pre-treated supernatant as a carbon source under nutrient-limiting condition. Moreover, the biogas generation of 0.187 L/g chemical oxygen demand was achieved using settled pretreated sludge. The pretreated sludge sample thus served as a carbon source for polyhydroxybutyrate producers as well as substrate for biogas production.

在这项研究中，开发了一种创新方法，通过相分离预处理来增强水解，方法是通过温和的超声处理去除胞外聚合物，然后进行热芬顿分解。在 2.58 kJ/kg 总固体的声波比能输入下，胞外聚合物的碎裂得到增强。去除胞外聚合物后，与热芬顿相比，在 Fe ²⁺ :H ₂ O ₂剂量和 0.009:0.036 g/g 悬浮固体和 80 °C 的温度下，热芬顿对生物质的分解产生 29.8% 的溶解度孤独解体。93.1 % 的聚羟基丁酸酯含量由Bacillus aryabhatai积累在 42 小时的最佳时间，同时提供 70% (v/v) 预处理的上清液作为营养限制条件下的碳源。此外，使用沉淀的预处理污泥实现了 0.187 L/g 化学需氧量的沼气产生。因此，经过预处理的污泥样品可作为聚羟基丁酸酯生产商的碳源以及沼气生产的底物。