In the present study, paclitaxel (PTX), multi-walled carbon nanotubes (MWCNTs), and doxorubicin (DOX) have been simultaneously doped into the poly(ϵ-caprolactone) (PCL)/chitosan/zein core-shell nanofibers to increase its cytotoxicity for MCF-7 breast cancers killing. The physico-chemical properties of synthesized nanofibers were determined by scanning electron microscope, Fourier-transform infrared spectroscopy, tensile strength, and degradation rate determinations. The in vitro release studies demonstrated the sustained release of drugs from core-shell nanofibrous scaffold. The cytotoxicity and compatibility of core-shell nanofibers were investigated by their treating with MCF-7 breast cancer cells and L929 normal cells, respectively. PCL/PTX/chitosan/zein/MWCNTs/DOX core-shell nanofibers containing 1 wt% MWCNTs, 100 μg ml⁻¹ DOX and 100 μg ml⁻¹ PTX had a high biocompatibility with a 84% MCF-7 cancer cells killing. The in vivo studies revealed the synergic effects of MWCNTs and anticancer drugs on the tumor inhibition. This method could be considered as a new way for developing of MWCNTs loaded-nanofibers for cancer treatment in future.

中文翻译：

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制备聚（ε-己内酯）/紫杉醇（核心）/壳聚糖/玉米醇溶蛋白/多壁碳纳米管/多柔比星（壳）纳米纤维对抗 MCF-7 乳腺癌

在本研究中，紫杉醇 (PTX)、多壁碳纳米管 (MWCNT) 和阿霉素 (DOX) 同时掺杂到聚 (ε-己内酯) (PCL)/壳聚糖/玉米醇溶蛋白核壳纳米纤维中，以提高其MCF-7 乳腺癌杀伤的细胞毒性。通过扫描电子显微镜、傅里叶变换红外光谱、拉伸强度和降解率测定来测定合成纳米纤维的物理化学性质。体外释放研究证明了药物从核-壳纳米纤维支架中的持续释放。通过分别处理 MCF-7 乳腺癌细胞和 L929 正常细胞，研究了核-壳纳米纤维的细胞毒性和相容性。PCL/PTX/壳聚糖/玉米醇溶蛋白/MWCNTs/DOX 核-壳纳米纤维，含 1 wt% MWCNTs，100 μg ml^-1 DOX 和 100 μg ml ^-1 PTX 具有高生物相容性，可杀死 84% 的 MCF-7 癌细胞。体内研究揭示了多壁碳纳米管和抗癌药物对肿瘤抑制的协同作用。该方法可被视为未来开发用于癌症治疗的载有多壁碳纳米管的纳米纤维的新途径。