We developed and validated an optimized method for quantifying lignans using ultra-performance liquid chromatography (UPLC) and performs correlation analysis of growth characteristics and contents of lignans. The methods for determining lignans were validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision using UPLC. All calibration curves showed good linearity (r2 ≥ 0.9998) within the tested ranges. The limit of detection (LOD) and limit of quantification (LOQ) were 0.003–0.02 µg/mL and 0.01–0.07 µg/mL, respectively. The precision of analysis was less than 3%. The recoveries of quantified compounds ranged from 98.4 to 101.68%. Growth characteristics of fruits were negatively correlated with content of total marker compounds. The results of this study can be used to quality-control of S. chinensis fruits used as medicinal raw materials.

中文翻译：

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不同栽培地区五味子果实生长特性及木酚素含量

我们开发并验证了一种使用超高效液相色谱 (UPLC) 量化木脂素的优化方法，并对木脂素的生长特性和含量进行了相关分析。通过使用 UPLC 测量线性、检测限 (LOD)、定量限 (LOQ)、准确度和精密度来验证木脂素的测定方法。所有校准曲线在测试范围内均显示出良好的线性 (r2 ≥ 0.9998)。检测限 (LOD) 和定量限 (LOQ) 分别为 0.003–0.02 µg/mL 和 0.01–0.07 µg/mL。分析精度小于3%。定量化合物的回收率为 98.4 至 101.68%。果实生长特性与总标记物含量呈负相关。本研究结果可用于S的质量控制。