Deciphering ecological functions of alkaline phosphatase (phoD)-harboring bacteria in composting systems is crucial but poorly understood. High-throughput sequencing, gene quantification, and statistical analyses were applied to investigate effects of abundance and diversity of phoD-harboring bacteria (PHB) on phosphorus availability during swine manure composting. Results showed that available phosphorus notably increased from 0.5 to 1.43 g kg⁻¹, and physicochemical properties and enzyme activities affected PHB community composition. Phylogenetic signals of PHB responded notably to temperature and phosphorus components, and stochasticity (94.2 %) dominated community assembly. Abundance and diversity of PHB directly and indirectly influenced phosphorus availability, and rare PHB mediated organic phosphorus mineralization. A phosphate-solubilizing bacterium (PSB) Pseudomonas sp. WWJ-22 isolated from compost displayed good efficiency in mineralizing lecithin, demonstrating the highest phosphorus-solubilizing level of 116.3 mg L^–1. This study highlights ecological roles of PHB on phosphorus availability and provides a potential PSB candidate for composting.

破译堆肥系统中碱性磷酸酶 ( phoD ) 携带细菌的生态功能至关重要，但人们知之甚少。应用高通量测序、基因定量和统计分析来研究phoD携带细菌 (PHB) 的丰度和多样性对猪粪堆肥过程中磷有效性的影响。结果表明，有效磷从 0.5 显着增加到 1.43 g kg ⁻¹，理化性质和酶活性影响 PHB 群落组成。PHB 的系统发育信号对温度和磷成分反应显着，随机性 (94.2%) 主导群落组装。PHB 的丰度和多样性直接和间接影响磷的有效性，以及稀有 PHB 介导的有机磷矿化。磷酸盐溶解细菌 (PSB)假单胞菌属。从堆肥中分离出的 WWJ-22 在矿化卵磷脂方面表现出良好的效率，表明最高的磷溶解水平为 116.3 mg L ^–1。本研究强调了 PHB 对磷可用性的生态作用，并为堆肥提供了潜在的 PSB 候选物。