This study established an integrated loach-plant-substrate-microbes non-aerated saturated vertical flow constructed wetlands (VFCWs) to enhance pollutants removal efficiencies and reduce greenhouse gas emissions simultaneously. The results of the VFCWs experiment indicated that the removal efficiencies of chemical oxygen demand, total phosphorous, and total nitrogen in loach systems were significantly higher than those of non-loach systems, achieving 59.16%, 35.98%, and 40.96%, respectively. The CH₄ and N₂O emission fluxes were also significantly reduced in the integrated system, resulting in lower global warming potential (GWP) and GWP per unit of pollutants removal. Loaches promoted the transportation of oxygen, facilitated the re-contact and utilization of sediments, reduced CH₄ emission, and enhanced nitrogen conversion and phosphorus accumulation. Increased bioavailable carbon and nitrate-nitrogen in the integrated system improved the abundance of denitrifying bacteria, which supported complete denitrification, reducing N₂O emissions with high pollutant removal.

本研究建立了泥鳅-植物-基质-微生物一体化非曝气饱和垂直流人工湿地（VFCWs），以提高污染物去除效率，同时减少温室气体排放。VFCWs实验结果表明，泥鳅系统对化学需氧量、总磷和总氮的去除效率显着高于非泥鳅系统，分别达到59.16%、35.98%和40.96%。CH ₄和N ₂ O排放通量在集成系统中也显着降低，导致全球变暖潜能值（GWP）和每单位污染物去除的GWP降低。泥鳅促进氧气的运输，促进沉积物的再接触和利用，减少CH₄排放，增强氮转化和磷积累。集成系统中生物可利用碳和硝态氮的增加提高了反硝化细菌的丰度，支持完全反硝化，减少 N ₂ O 排放，同时去除污染物。