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Development of an LC/MS/MS Method for Simultaneous Detection of 11 Polyphenols in Rat Plasma and Its Pharmacokinetic Application after Oral Administration of Coreopsis tinctoria Extract
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2022-11-16 , DOI: 10.1002/cbdv.202200574 Haixia Han 1 , Rui Ma 1 , Aidi Xie 1 , Juanjuan Gao 1 , Zhen Wang 1 , Yi Zhao 1 , Huanming Pang 1 , Wei Zhang 2
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2022-11-16 , DOI: 10.1002/cbdv.202200574 Haixia Han 1 , Rui Ma 1 , Aidi Xie 1 , Juanjuan Gao 1 , Zhen Wang 1 , Yi Zhao 1 , Huanming Pang 1 , Wei Zhang 2
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Eleven polyphenols, classified as flavonoid glycosides, flavonoid aglycones, and phenolic acids, are important bioactive components in the capitula of Coreopsis tinctoria (CCT). Nevertheless, their full pharmacokinetic profiles have not been demonstrated simultaneously. Therefore, a liquid chromatography – tandem mass spectrometry (LC/MS/MS) method was developed in the present work and used it to study the pharmacokinetics of these 11 compounds. We performed LC/MS/MS with a gradient mobile phase composed of water containing 0.1 % formic acid and acetonitrile containing 0.1 % formic acid on a Proshell 120 SB C18 column (2.1 mm×100 mm, 2.7 μm). We achieved a good chromatographic peak shape, resolution, and mass signal response, and multiple reaction monitoring facilitated the simultaneous detection of 11 analytes. In addition, we validated the selectivity, correlation coefficient, precision, extraction recovery, matrix effects, and stability of the LC/MS/MS method to be acceptable for 11 analytes in rat plasma. Subsequently, rats were orally administered with 50 % ethanol eluent of CCT (ECCT). Nine of 11 polyphenols were absorbed quickly (except for QCD and TCA), and their plasma levels peaked within 40 min. The exposure and Cmax values of flavonoid glycosides and phenolic acids were lower than those of flavonoid aglycones. This is the first report to demonstrate the pharmacokinetics of 11 polyphenols in ECCT, which may play an important role in future studies of the bioactive components of ECCT and their bioactive mechanisms.
中文翻译：
建立同时检测大鼠血浆中 11 种多酚的 LC/MS/MS 方法及其在口服金鸡菊提取物后的药代动力学应用
黄酮苷类、黄酮苷元类和酚酸类的 11 种多酚是金鸡菊头状花序中重要的生物活性成分（中转）。然而，它们的完整药代动力学特征尚未同时得到证实。因此，本工作开发了一种液相色谱-串联质谱 (LC/MS/MS) 方法，用于研究这 11 种化合物的药代动力学。我们在 Proshell 120 SB C18 色谱柱（2.1 mm×100 mm，2.7 μm）上使用由含 0.1% 甲酸的水和含 0.1% 甲酸的乙腈组成的梯度流动相进行 LC/MS/MS。我们实现了良好的色谱峰形、分辨率和质量信号响应，多反应监测有助于同时检测 11 种分析物。此外，我们验证了 LC/MS/MS 方法的选择性、相关系数、精密度、提取回收率、基质效应和稳定性对于大鼠血浆中的 11 种分析物是可接受的。随后，给大鼠口服 50% 的 CCT (ECCT) 乙醇洗脱液。11 种多酚中有 9 种被快速吸收（QCD 和 TCA 除外），它们的血浆水平在 40 分钟内达到峰值。曝光和黄酮苷和酚酸的C max值低于黄酮苷元。这是第一份证明ECCT中11种多酚药代动力学的报道，这可能对未来研究ECCT的生物活性成分及其生物活性机制起到重要作用。
更新日期：2022-11-16
中文翻译：
建立同时检测大鼠血浆中 11 种多酚的 LC/MS/MS 方法及其在口服金鸡菊提取物后的药代动力学应用
黄酮苷类、黄酮苷元类和酚酸类的 11 种多酚是金鸡菊头状花序中重要的生物活性成分（中转）。然而，它们的完整药代动力学特征尚未同时得到证实。因此，本工作开发了一种液相色谱-串联质谱 (LC/MS/MS) 方法，用于研究这 11 种化合物的药代动力学。我们在 Proshell 120 SB C18 色谱柱（2.1 mm×100 mm，2.7 μm）上使用由含 0.1% 甲酸的水和含 0.1% 甲酸的乙腈组成的梯度流动相进行 LC/MS/MS。我们实现了良好的色谱峰形、分辨率和质量信号响应，多反应监测有助于同时检测 11 种分析物。此外，我们验证了 LC/MS/MS 方法的选择性、相关系数、精密度、提取回收率、基质效应和稳定性对于大鼠血浆中的 11 种分析物是可接受的。随后，给大鼠口服 50% 的 CCT (ECCT) 乙醇洗脱液。11 种多酚中有 9 种被快速吸收（QCD 和 TCA 除外），它们的血浆水平在 40 分钟内达到峰值。曝光和黄酮苷和酚酸的C max值低于黄酮苷元。这是第一份证明ECCT中11种多酚药代动力学的报道，这可能对未来研究ECCT的生物活性成分及其生物活性机制起到重要作用。
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