Transcription factors (TFs) can manage the coordinated expression of genes clusters or multiple genes. TF was used to improve bacterial denitrification ability in this study. During denitrification, the ntrY of R. azotoformans, which encodes the sensor of NtrX/Y system, was significantly upregulated in transcription. Denitrification of the mutant △ntrY was significantly inhibited, and it was recovered after replenishing this gene to the mutant, which indicates the NtrX/Y system plays an important role in regulating bacterial denitrification. According to additional research, the NtrX/Y system regulates bacterial denitrification by directly promoting the expression of the nitrite reductase. ntrY overexpression appears to accelerate bacterial denitrification, and the introduction of a strong promoter tac in conjunction with iron supply optimization increases the rate by 72% further. This study realizes bacterial denitrification enhancement from the perspective of global transcription regulation, which provides a novel strategy for improving microbial ability to degrade pollutants.

转录因子 (TF) 可以管理基因簇或多个基因的协调表达。TF在本研究中用于提高细菌反硝化能力。在反硝化过程中，编码NtrX /Y 系统传感器的偶氮菌的 ntrY 在转录中显着上调。突变体△ ntrY的反硝化作用受到显着抑制，向突变体补充该基因后反硝化作用得以恢复，说明NtrX/Y系统在调控细菌反硝化过程中发挥重要作用。根据其他研究，NtrX/Y 系统通过直接促进亚硝酸还原酶的表达来调节细菌反硝化作用。数过表达似乎加速了细菌反硝化作用，引入强启动子tac并结合铁供应优化进一步提高了 72% 的速率。该研究从全局转录调控的角度实现了细菌反硝化作用的增强，为提高微生物降解污染物的能力提供了一种新策略。