Composited carbon nanomaterials have attracted wide attention and are used for high-sensitivity biological assays due to their low toxicity, good biocompatibility, and excellent electrical conductivity. To further promote electron transfer and enhance electrocatalytic activity to detect dopamine (DA), this study proposed carbon dots (CDs) based on glycerol synthesized by liquid dielectric barrier discharge (DBD) microplasma. Combined with the multi-walled carbon nanotubes (MWCNTs) with excellent electrical conductivity, a composited carbon nanomaterial electrode of CDs/MWCNTs was constructed. As a DA biosensor, the interaction and electron exchange between MWCNTs, CDs, and DA can be enhanced thanks to the π-π stacking force, thereby facilitating the sensitive electrochemical detection of DA. The sensor exhibits good sensing performance toward DA detection with a linear range of 2.0–100 μM, a limit of detection (LOD) of 11.08 nM (S/N = 3), and a sensitivity of 29020 μA cm⁻² mM⁻¹. The proposed electrode successfully detected DA levels in human serum samples with satisfactory selectivity and recovery rate. The microplasma-enabled synthesized method provides a promising path for preparing and applying carbon-based nanomaterials.

复合碳纳米材料因其低毒性、良好的生物相容性和优异的导电性而受到广泛关注，并被用于高灵敏度的生物检测。为了进一步促进电子转移并增强检测多巴胺 (DA) 的电催化活性，本研究提出了基于液体介质阻挡放电 (DBD) 微等离子体合成的甘油的碳点 (CD)。结合具有优异导电性的多壁碳纳米管（MWCNTs），构建了CDs/MWCNTs复合碳纳米材料电极。作为 DA 生物传感器，由于 π-π 堆积力，可以增强 MWCNT、CD 和 DA 之间的相互作用和电子交换，从而促进 DA 的灵敏电化学检测。^-2 毫米^-1。所提出的电极成功地检测了人血清样品中的 DA 水平，具有令人满意的选择性和回收率。微等离子体合成方法为碳基纳米材料的制备和应用提供了一条有前途的途径。