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Enrichment and Distribution of Selenium in Pediococcus acidilactici MRS-7: Impact on Its Biochemical Composition, Microstructure, and Gastrointestinal Survival
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2022-11-16 , DOI: 10.1021/acs.jafc.2c06765
Hong Chen 1, 2 , Xiaoyu Wang 1, 2 , Yuan Yue 3 , Xin Wang 1, 2 , Xuejun Zeng 1, 2 , Qi Guo 1, 2 , Xiaohai Yan 1, 2 , Gengan Du 1, 2 , Yahong Yuan 1, 2 , Tianli Yue 1, 2, 4
Affiliation  

Lactic acid bacteria can convert selenium (Se) from inorganic to organic and elemental forms, but the distribution and existence form of organic Se in the bacteria are not clear after Se enrichment, and the effects of selenization on the growth and nutritional value of strains also need to be studied. In this study, Pediococcus acidilactici MRS-7 could absorb up to 67% of inorganic Se and convert most of it into organic Se; about 75% of organic Se was selenoprotein, 2.7% was Se-polysaccharide, and 4.6% was Se-nucleic acid. Additionally, Se-enriched treatment increased the levels of amino acids and essential elements in P. acidilactici MRS-7. Finally, after Se enrichment, Se nanoparticles (SeNPs) were found on the surface of P. acidilactici MRS-7, but they had no harmful effect on its morphology, and its survival during gastrointestinal digestion was not affected, indicating that SeP has potential probiotic value in the food industry.

中文翻译:

乳酸片球菌 MRS-7 中硒的富集和分布:对其生化成分、微观结构和胃肠道存活的影响

乳酸菌可以将硒(Se)从无机态转化为有机态和单质态,但富硒后有机硒在菌体内的分布和存在形式尚不清楚,硒化对菌株生长和营养价值的影响也需要研究。在这项研究中,Pediococcus acidilactici MRS-7 可以吸收高达 67% 的无机硒并将其中的大部分转化为有机硒;有机硒中约75%为硒蛋白,2.7%为硒多糖,4.6%为硒核酸。此外,富硒处理提高了P. acidilactici MRS-7中氨基酸和必需元素的水平。最后,在 Se 富集后,在P. acidilactici的表面发现了 Se 纳米颗粒 (SeNPs)MRS-7,但对其形态无不良影响,其在胃肠道消化过程中的存活不受影响,表明SeP在食品工业中具有潜在的益生菌价值。
更新日期:2022-11-16
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