Abstract

Two new tigliane- and daphnane-type diterpenoids, given the trivial names daphnegens A–B (1–2) were isolated from the buds of Daphne genkwa. Their structures were assigned on the basis of extensive spectroscopic. The absolute configurations of both compounds were determined by comparison of their calculated and experimental CD curves. In addition, compounds 1–2 were tested for their cytotoxic activities against MCF-7 and HepG-2 human cancer cell lines, and compound 2 showed remarkable cytotoxic activity against HepG-2 cell line with the IC₅₀ value of 11.5 μM.

中文翻译：

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芫花花蕾中的提利亚烷型和瑞香型二萜及其细胞毒活性

摘要

从Daphne genkwa的芽中分离出两种新的 tigliane 型和 daphnane 型二萜类化合物，俗称 daphnegens A–B (1 – 2)。它们的结构是根据广泛的光谱确定的。通过比较计算的CD曲线和实验的CD曲线来确定两种化合物的绝对构型。此外，还测试了化合物1 ~ 2对MCF-7和HepG-2人癌细胞系的细胞毒活性，其中化合物2对HepG-2细胞系表现出显着的细胞毒活性，IC ₅₀值为11.5  μM。