Abstract

Adipogenesis involves commitment of stem cells and their differentiation into mature adipocytes. It is tightly regulated by hormones, nutrients and adipokines. Many natural compounds are being tested for their anti-adipogenic activity which can be attributed to apoptosis induction in adipocytes, blocking adipocyte differentiation, or inhibiting intracellular triglyceride synthesis and accumulation. In this study, we have determined molecular mechanism of two phytocompounds: andrographolide (AN) and pterostilbene (PT) during differentiation of the human MSCs into adipocyte. Interestingly, AN upregulates miR27a, whereas, PT upregulated SIRT1 which inhibits the expression of PPARγ. Thus, our results clearly demonstrate that both AN and PT inhibited adipogenesis by blocking a surge of reactive oxygen species (ROS) during differentiation and inhibiting expression of crucial transcription factors like SREBP1c and PPARγ.

摘要

脂肪生成涉及干细胞的定型及其分化为成熟脂肪细胞。它受到激素、营养素和脂肪因子的严格调节。正在测试许多天然化合物的抗脂肪形成活性，该活性可归因于诱导脂肪细胞凋亡、阻断脂肪细胞分化或抑制细胞内甘油三酯的合成和积累。在这项研究中，我们确定了两种植物化合物：穿心莲内酯（AN）和紫檀芪（PT）在人间充质干细胞分化为脂肪细胞过程中的分子机制。有趣的是，AN 上调 miR27a，而 PT 上调 SIRT1，后者抑制 PPARγ 的表达。因此，我们的结果清楚地表明，AN 和 PT 都通过阻断分化过程中活性氧 (ROS) 的激增以及抑制 SREBP1c 和 PPARγ 等关键转录因子的表达来抑制脂肪生成。