A simultaneous analytical method has been developed for quantification and confirmation of the nematicide fluazaindolizine and its seven metabolites (IN-A5760, IN-F4106, IN-QEK31, IN-QZY47, IN-TMQ01, IN-UNS90 and IN-UJV12) in agricultural products. The compounds were extracted with acetonitrile/water (80/20, v/v) and purified using C18 cartridge, and analysis was conducted by liquid chromatography-tandem mass spectrometry in the electrospray positive and negative ion mode. The method has been validated by verifying the performance characteristics such as selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision. To prevent the matrix effects, all analytes were quantified with matrix-matched calibration assessed by the determination coefficient (R2) of the range from 0.9988 to 1.0000. The LOD and LOQ were satisfactory to determine the low residual level in agricultural products. The accuracy and precision of the method were evaluated by recoveries with five replicates at three fortification levels (LOQ, 10 × LOQ and 50 × LOQ). The mean recoveries of fluazaindolizine and seven metabolites in agricultural products were 75.6–110.0% with the CV% of 0.2–9.1%. All optimized results were displayed excellent results assessed by the Ministry of Food and Drug Safety guidelines and the Codex Alimentarius Commission guidelines for pesticide residue analysis. This study could use as basic data for setting of residue definition and maximum residue limits of fluazaindolizine in agricultural products.

中文翻译：

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液相色谱串联质谱法同时测定农产品中氟氮唑嗪及其7种代谢物的分析方法的建立

开发了一种同时分析方法，用于定量和确认农业中杀线虫剂氟氮唑嗪及其七种代谢物（IN-A5760、IN-F4106、IN-QEK31、IN-QZY47、IN-TMQ01、IN-UNS90 和 IN-UJV12）产品。化合物用乙腈/水（80/20，v/v）萃取，C18柱纯化，液相色谱-串联质谱电喷雾正负离子模式进行分析。该方法已通过验证选择性、线性、检测限 (LOD)、定量限 (LOQ)、准确度和精密度等性能特征得到验证。为防止基质效应，所有分析物均采用基质匹配校准进行量化，该校准由 0.9988 至 1.0000 范围内的决定系数 (R2) 评估。LOD 和 LOQ 令人满意地确定了农产品中的低残留水平。该方法的准确度和精密度通过三个加标水平（LOQ、10 × LOQ 和 50 × LOQ）的 5 次重复的回收率进行评估。农产品中氟氮唑嗪和七种代谢物的平均回收率为 75.6-110.0%，CV% 为 0.2-9.1%。所有优化结果均显示出食品和药品安全部指南和食品法典委员会农药残留分析指南评估的优异结果。本研究可作为确定农产品中氟氮唑嗪残留量定义和最大残留限量的基础数据。该方法的准确度和精密度通过三个加标水平（LOQ、10 × LOQ 和 50 × LOQ）的 5 次重复的回收率进行评估。农产品中氟氮唑嗪和七种代谢物的平均回收率为 75.6-110.0%，CV% 为 0.2-9.1%。所有优化结果均显示出食品和药品安全部指南和食品法典委员会农药残留分析指南评估的优异结果。本研究可作为确定农产品中氟氮唑嗪残留量定义和最大残留限量的基础数据。该方法的准确度和精密度通过三个加标水平（LOQ、10 × LOQ 和 50 × LOQ）的 5 次重复的回收率进行评估。农产品中氟氮唑嗪和七种代谢物的平均回收率为 75.6-110.0%，CV% 为 0.2-9.1%。所有优化结果均显示出食品和药品安全部指南和食品法典委员会农药残留分析指南评估的优异结果。本研究可作为确定农产品中氟氮唑嗪残留量定义和最大残留限量的基础数据。所有优化结果均显示出食品和药品安全部指南和食品法典委员会农药残留分析指南评估的优异结果。本研究可作为确定农产品中氟氮唑嗪残留量定义和最大残留限量的基础数据。所有优化结果均显示出食品和药品安全部指南和食品法典委员会农药残留分析指南评估的优异结果。本研究可作为确定农产品中氟氮唑嗪残留量定义和最大残留限量的基础数据。