Abstract

An immobilized enzyme could exhibit selectively modified physicochemical properties, and it might offer a better environment for the enzyme activity. In this study, the immobilization yield of crude Halomonas sp. lipase was optimized to improve its stability. Thanks to its high adsorption capacity, CaCO₃ has been chosen as support for the immobilization process. Furthermore, response surface methodology (RSM) was used to determine optimal conditions for the immobilization of the bacterial lipase. Five tested factors (enzyme solution, support amount, time, temperature, and acetone volume) were optimized applying a central composite design of RSM. The maximum yield of lipase immobilization was improved to 96%. Furthermore, a biochemical characterization proved a significant improvement of the immobilized lipase stability. The immobilized enzyme is more stable at extreme pH values and high temperatures than the free one. We also tested the reusability of the immobilized lipase by evaluating the recovery of the support using simple filtration. Thanks to its high stability, the immobilized lipase was invested in an effective treatment of tuna wash processing wastewater. The oil biodegradation efficiency was established at 81.5% and was confirmed by Fourier transformation infrared spectrometry. Likewise, the biological oxygen demand values were reduced which makes a possible reduction of the wastewater pollution degree.

摘要

固定化酶可以表现出选择性修饰的理化性质，并且可以为酶活性提供更好的环境。在本研究中，粗单胞菌的固定化产量。对脂肪酶进行了优化以提高其稳定性。由于其高吸附能力，CaCO ₃被选为固定化过程的载体。此外，响应面法（RSM）用于确定细菌脂肪酶固定的最佳条件。应用 RSM 的中心复合设计对五个测试因素（酶溶液、支持量、时间、温度和丙酮体积）进行了优化。脂肪酶固定化率最大提高至96%。此外，生化表征证明固定化脂肪酶稳定性显着提高。固定化酶在极端 pH 值和高温下比游离酶更稳定。我们还通过使用简单过滤评估支持物的回收率来测试固定化脂肪酶的可重复使用性。由于其高稳定性，固定化脂肪酶被用于有效处理金枪鱼清洗加工废水。油生物降解效率确定为 81.5%，并通过傅里叶变换红外光谱法证实。同样，生物需氧量值也降低，从而可能降低废水污染程度。