It was critically important to develop some sensitive, rapid, and specific imaging or detection methods for the virulent strain in food safety monitoring. In the study, a novel tetraphenyl mono-phenylboronic acid dye (TPE-PBA) with good aggregation-induced emission (AIE) features and high combining capacity towards bacteria was first synthesized. With TPE-PBA as a signal tag, a sandwich-type AIE probe-linked phage sorbent assay was developed for imaging and detecting virulent strains using Escherichia coli O157:H7 (E. coli O157:H7) as a representative. In the assay, phages for E. coli O157:H7 were firstly fixed on the bottom of a 96-well plate to specifically capture the strain, then the TPE-PBA signal tag was added and incubated with the captured strain to produce the phage/E. coli O157:H7/TPE-PBA complex. The complex could produce intensive AIE fluorescence being proportional to the amount of E. coli O157:H7 with a detection limit of 30 CFU mL⁻¹ within 30 min. Simultaneously, the strain could be imaged in the plate with good anti-photobleaching and AIE effects. The results demonstrated the AIE-linked phage sorbent assay with a TPE-PBA signal tag could provide a suitable platform for rapid and specific detection and imaging of virulent strains. Therefore, it exhibited good application prospects in the on-site monitoring of food pathogens.

在食品安全监测中开发一些灵敏、快速和特异性的强毒株成像或检测方法至关重要。在该研究中，首次合成了一种具有良好聚集诱导发光（AIE）特性和对细菌具有高结合能力的新型四苯基单苯基硼酸染料（TPE-PBA）。以TPE-PBA为信号标签，以大肠杆菌O157:H7（大肠杆菌O157:H7）为代表，开发了夹心型AIE探针连接的噬菌体吸附剂，用于成像和检测强毒株。在测定中，大肠杆菌的噬菌体O157:H7先固定在96孔板底部特异性捕获菌株，然后加入TPE-PBA信号标签，与捕获菌株孵育产生噬菌体/大肠杆菌O157:H7/TPE- PBA 复合体。该复合物可产生与大肠杆菌O157:H7量成正比的强烈 AIE 荧光，检测限为 30 CFU mL ^-130 分钟内 同时，应变可以在板中成像，具有良好的抗光漂白和AIE效果。结果表明，带有 TPE-PBA 信号标签的 AIE 相关噬菌体吸附剂测定可为强毒株的快速特异性检测和成像提供合适的平台。因此，它在食品致病菌的现场监测中表现出良好的应用前景。