Transcription factors (TFs) play important roles in regulating multiple biological processes by binding to promoter regions and regulating the global gene transcription levels. Pseudomonas syringae is a gram-negative phytopathogenic bacterium harbouring 301 putative TFs in its genome, approximately 50 of which are responsible for virulence-related gene and pathway regulation. Over the past decades, RNA sequencing, chromatin immunoprecipitation sequencing, high-throughput systematic evolution of ligands by exponential enrichment, and other technologies have been applied to identify the functions of master regulators and their interactions in virulence-related pathways. This review summarises the recent advances in the regulatory networks of TFs involved in the type III secretion system (T3SS) and non-T3SS virulence-associated pathways, including motility, biofilm formation, quorum sensing, nucleotide-based secondary messengers, phytotoxins, siderophore production, and oxidative stress. Moreover, this review discusses the future perspectives in terms of TF-mediated pathogenesis mechanisms and provides novel insights that will help combat P. syringae infections based on the regulatory networks of TFs.

转录因子（TF）通过与启动子区域结合并调节全局基因转录水平，在调节多种生物过程中发挥重要作用。丁香假单胞菌是一种革兰氏阴性植物病原细菌，其基因组中含有 301 个假定的转录因子，其中大约 50 个负责毒力相关基因和途径调控。在过去的几十年里，RNA测序、染色质免疫沉淀测序、通过指数富集进行配体的高通量系统进化以及其他技术已被应用于鉴定主调节因子的功能及其在毒力相关途径中的相互作用。本综述总结了涉及 III 型分泌系统 (T3SS) 和非 T3SS 毒力相关途径的 TF 调控网络的最新进展，包括运动性、生物膜形成、群体感应、基于核苷酸的第二信使、植物毒素、铁载体产生和氧化应激。此外，这篇综述讨论了 TF 介导的发病机制的未来前景，并提供了新的见解，将有助于基于 TF 调控网络对抗丁香假单胞菌感染。