The most important environmental factor that can play a key role in the development of asthma in the obese is overproduction of reactive oxygen species (ROS). The aim of the study was to examine changes in the concentration of oxidative stress parameters in the lungs, bronchoalveolar lavage (BAL) fluid and blood of mice in models of asthma or/and obesity caused by high-fat diet (HFD). The concentrations of 4-HNE and isoprostanes in the lungs of the animals were measured. BAL fluid levels of hydrogen peroxide were marked. Additionally, thiobarbituric acid reactive substances (TBARS) and ferric reducing ability of plasma (FRAP) were used as biomarkers of oxidative stress in the blood. Administration of lipoic acid (LA), a probiotic with standard-fat diet (SFD, 10% fat) and low-fat diet (LFD, 5% fat) significantly decreased the concentration of 4-HNE as compared to the OVA (ovalbumin) + HFD group (p < 0.05). Treatment with low-fat diet or LFD in combination with apocynin insignificantly decreased H₂O₂ values as compared to the OVA + HFD group. Supplementation of probiotic with SFD and LFD significantly decreased the concentration of TBARS as compared to the OVA + SFD and saline + HDF groups (p < 0.05). Significantly lower concentrations of TBARS were also observed in the LA plus LFD group (p < 0.05) as compared to the OVA + HFD group. Low-fat diet with probiotic significantly increased the concentration of FRAP as compared to the obese mice (p = 0.017). Treatment with LFD in combination with LA significantly increased FRAP values as compared to the obese and obese asthmatic mice (p < 0.001).

在肥胖者哮喘的发展中起关键作用的最重要的环境因素是活性氧 (ROS) 的过量产生。该研究的目的是检查在高脂肪饮食 (HFD) 引起的哮喘或/和肥胖模型中小鼠的肺、支气管肺泡灌洗液 (BAL) 液和血液中氧化应激参数浓度的变化。测量了动物肺中 4-HNE 和异前列烷的浓度。标记了过氧化氢的 BAL 液体水平。此外，硫代巴比妥酸反应物质 (TBARS) 和血浆铁还原能力 (FRAP) 被用作血液中氧化应激的生物标志物。使用标准脂肪饮食（SFD，10% 脂肪）和低脂肪饮食（LFD，p  < 0.05）。与 OVA + HFD 组相比，低脂饮食或 LFD 与夹竹桃麻素的组合治疗没有显着降低 H ₂ O _2值。与 OVA + SFD 和盐水 + HDF 组相比，用 SFD 和 LFD 补充益生菌显着降低了 TBARS 的浓度 ( p  < 0.05)。 与 OVA + HFD 组相比，在 LA 加 LFD 组中也观察到显着较低浓度的 TBARS ( p < 0.05)。与肥胖小鼠相比，含益生菌的低脂饮食显着增加了 FRAP 的浓度 ( p  = 0.017)。与肥胖和肥胖哮喘小鼠相比，LFD 联合 LA 治疗显着增加了 FRAP 值（p  < 0.001）。