Abstract

Autophagy is a protective mechanism important in human diseases as cancer. We evaluated the impact of khalas date extract (KDE) (20-60 mg/mL) on cell viability, morphological changes, DNA fragmentation and gene expression of LC3B-II associated with autophagosome on HepG2 cell line. The GC/MS identification of KDE showed its high content of flavonoids including quercetin, myricetin, kaempferol and catechol. KDE reduced cell viability of HepG2 with IC₅₀ (31.52 mg/mL). Cells treated with KDE showed two band of DNA fragments at (30 and 40 mg) indicating that KDE induced DNA damage and apoptosis in HepG2. The analysis RT-PCR data showed a 0.2-fold increase in the expression of LC3-B in the cells treated with KDE versus control. We concluded that, KDE flavonoids such as quercetin, myricetin kaempferol exhibited anticancer properties manifested by inhibition of HepG2 cell viability and induction of apoptosis and upregulation of the pro-autophagy LC3-B gene.

摘要

自噬是癌症等人类疾病中重要的保护机制。我们评估了哈拉斯枣提取物 (KDE) (20-60 mg/mL) 对 HepG2 细胞系上细胞活力、形态变化、DNA 碎片以及与自噬体相关的 LC3B-II 基因表达的影响。KDE的GC/MS鉴定表明其含有较高含量的黄酮类化合物，包括槲皮素、杨梅素、山奈酚和儿茶酚。KDE 降低了 HepG2 的细胞活力，IC 为₅₀ (31.52 mg/mL)。用 KDE 处理的细胞在（30 和 40 mg）显示两条 DNA 片段带，表明 KDE 诱导 HepG2 中的 DNA 损伤和细胞凋亡。RT-PCR 分析数据显示，与对照相比，用 KDE 处理的细胞中 LC3-B 的表达增加了 0.2 倍。我们得出的结论是，KDE 黄酮类化合物（例如槲皮素、杨梅素、山奈酚）具有抗癌特性，具体表现在抑制 HepG2 细胞活力、诱导细胞凋亡和上调促自噬 LC3-B 基因。