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A reinforced PDMS mold for hot embossing of cyclic olefin polymer in the fabrication of microfluidic chips
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-11-07 , DOI: 10.1039/d2lc00857b Yuling Qin 1 , Jason E Kreutz 2 , Thomas Schneider 2 , Gloria S Yen 2 , Eleanor S Shah 2 , Li Wu 1 , Daniel T Chiu 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-11-07 , DOI: 10.1039/d2lc00857b Yuling Qin 1 , Jason E Kreutz 2 , Thomas Schneider 2 , Gloria S Yen 2 , Eleanor S Shah 2 , Li Wu 1 , Daniel T Chiu 2
Affiliation
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Hot embossing is a cost-effective and flexible fabrication technology with high replication accuracy for feature sizes as small as 50 nm. Here we develop a reinforced polydimethylsiloxane (PDMS) mold for hot embossing of cyclic olefin polymer (COP) sheets in the fabrication of microfluidic chips and demonstrate the method by fabricating chips for automated sample digitization in digital nucleic acid assays. The PDMS is hardened by adding an investment powder as a dopant and is constrained with an aluminum frame to prevent lateral expansion during hot pressing. The reinforced PDMS mold demonstrated excellent performance in hot embossing (180 °C, 103 kPa, 5 min) for micropatterning COP sheets, with highly reproducible features as small as 10 μm (width of draining channel). In contrast, the microscale features were inconsistent and distorted when omitting either the investment powder or frame from the PDMS mold. COP chips were assembled by thermally bonding patterned and unpatterned COP sheets. We tested the performance of the COP chip for automated sample digitization in a digital LAMP assay used to quantify human papillomavirus-18 (HPV-18) DNA. A mixture of nucleic acid amplification reagents was loaded into the main channel of the chip using a syringe pump, then the solution was spontaneously partitioned into chambers (∼0.6 nL), which were then isolated by flowing oil through the chip. The digital LAMP assay produced accurately absolute quantitation of DNA at concentrations ranging from 10 to 1000 copies per μL. The strategy presented here provides a simple, low-cost method to prepare molds for hot embossing, which facilitates rapid validation of microfluidic designs.
中文翻译：
用于微流控芯片制造中环烯烃聚合物热压印的增强 PDMS 模具
热压印是一种经济高效且灵活的制造技术，具有高复制精度，特征尺寸小至 50 nm。在这里，我们开发了一种增强型聚二甲基硅氧烷（PDMS）模具，用于在微流控芯片的制造中热压印环烯烃聚合物（COP）片材，并通过制造用于数字核酸测定中自动样品数字化的芯片来演示该方法。PDMS 通过添加熔模粉末作为掺杂剂来硬化，并用铝框架约束以防止热压过程中的横向膨胀。增强型 PDMS 模具在微图案 COP 片材的热压印（180 °C，103 kPa，5 分钟）中表现出优异的性能，具有小至 10 μm（排水通道宽度）的高度可重复性特征。相比之下，当从 PDMS 模具中省略熔模粉末或框架时，微观特征不一致且扭曲。COP 芯片通过热粘合图案化和未图案化的 COP 片材进行组装。我们测试了 COP 芯片在用于量化人乳头瘤病毒 18 (HPV-18) DNA 的数字 LAMP 测定中自动样本数字化的性能。使用注射泵将核酸扩增试剂的混合物加载到芯片的主通道中，然后溶液自发地分配到室中（〜0.6 nL），然后通过使油流过芯片来隔离室。数字 LAMP 测定可对浓度范围为每 μL 10 至 1000 个拷贝的 DNA 进行准确的绝对定量。这里提出的策略提供了一种简单、低成本的方法来准备热压花模具，有利于微流体设计的快速验证。
更新日期：2022-11-11
中文翻译：
用于微流控芯片制造中环烯烃聚合物热压印的增强 PDMS 模具
热压印是一种经济高效且灵活的制造技术，具有高复制精度，特征尺寸小至 50 nm。在这里，我们开发了一种增强型聚二甲基硅氧烷（PDMS）模具，用于在微流控芯片的制造中热压印环烯烃聚合物（COP）片材，并通过制造用于数字核酸测定中自动样品数字化的芯片来演示该方法。PDMS 通过添加熔模粉末作为掺杂剂来硬化，并用铝框架约束以防止热压过程中的横向膨胀。增强型 PDMS 模具在微图案 COP 片材的热压印（180 °C，103 kPa，5 分钟）中表现出优异的性能，具有小至 10 μm（排水通道宽度）的高度可重复性特征。相比之下，当从 PDMS 模具中省略熔模粉末或框架时，微观特征不一致且扭曲。COP 芯片通过热粘合图案化和未图案化的 COP 片材进行组装。我们测试了 COP 芯片在用于量化人乳头瘤病毒 18 (HPV-18) DNA 的数字 LAMP 测定中自动样本数字化的性能。使用注射泵将核酸扩增试剂的混合物加载到芯片的主通道中，然后溶液自发地分配到室中（〜0.6 nL），然后通过使油流过芯片来隔离室。数字 LAMP 测定可对浓度范围为每 μL 10 至 1000 个拷贝的 DNA 进行准确的绝对定量。这里提出的策略提供了一种简单、低成本的方法来准备热压花模具，有利于微流体设计的快速验证。
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