Developed herein is an aptasensing array based on liquid crystal (LC) for monitoring of tobramycin (TOB) antibiotic. The direction of LC molecules from vertical to a random status was induced by the conformational changes of the specific aptamer due to its selective interaction with the target. The dark view of the aptasensing platform changed to colorful through observation by a polarized light microscope that clarifies the TOB presence. The aptasensor is especially able to determine TOB in the linear concentration range of 0.005–600 pM with a limit of detection (LOD) as 0.0021 pM. The TOB values can be determined successfully in the milk and chicken egg samples that highlights the potential applicability of the designed aptasensor. The proposed sensing approach is facile, operator-independent, label-free, and ultra-sensitive, making it novel for developing real-time portable sensing devices for future.

本文开发了一种基于液晶 (LC) 的适体传感阵列，用于监测妥布霉素 (TOB) 抗生素。LC 分子从垂直状态到随机状态的方向是由特定适配体由于其与靶标的选择性相互作用而发生的构象变化引起的。通过澄清 TOB 存在的偏振光显微镜观察，aptasensing 平台的黑暗视图变为彩色。aptasensor 特别能够确定线性浓度范围为 0.005–600 pM 的 TOB，检测限 (LOD) 为 0.0021 pM。TOB 值可以在牛奶和鸡蛋样品中成功测定，这突出了所设计的适体传感器的潜在适用性。所提出的传感方法简单、独立于操作员、无标签且超灵敏，