Tetracycline is extensively used as an antibiotic in animal husbandry, and there arose an increase in antibiotic resistance genes in the environment, posing a threat to human health. Motivated by this, a magnetic molecularly imprinted material based on synergistic recognition (1 + 1>2) was constructed and coupled with high-performance liquid chromatography to detect ultra-trace tetracycline in complicated samples. In this case, the molecularly imprinted polymers were synthesized via a “one-pot” method and acted as recognition elements on the surface of silica-coated ferroferric oxide particles. Aptamers and β-cyclodextrin, as functional monomers, had a synergistic effect on the recognition of tetracycline and the synergistic recognition factor was 1.7. Meanwhile, the material exhibited high selectivity to tetracycline with an imprinting factor of 7.6. In addition, compared to being modified on the surface, the stability of the aptamers was effectively improved by cross-linking in the molecularly imprinted polymer framework. Relevant experimental conditions, such as buffers, concentration of magnesium ions and adsorption time, were optimized. As a result, the method showed a limit of detection of 1.0 μg L⁻¹ and the linearity range of 0.005–0.5 mg L⁻¹, as well as certain reproducibility and stability. Furthermore, when applied for the analysis of animal feed samples, a significant reduction of matrix interference was observed with satisfactory recoveries (85.0–111.5%), which emphasized the good accuracy and practicability of the established method. For these advantages, the proposed method represents a versatile and powerful tool for the separation and detection of small molecule compounds in complicated real samples.

四环素作为抗生素广泛应用于畜牧业，环境中抗生素耐药基因增多，对人类健康构成威胁。受此启发，构建了一种基于协同识别（1+1>2）的磁性分子印迹材料，并与高效液相色谱联用检测复杂样品中的超痕量四环素。在这种情况下，分子印迹聚合物通过“一锅法”合成，并作为二氧化硅包覆的四氧化三铁颗粒表面的识别元素。适体和β-环糊精作为功能单体对四环素的识别具有协同作用，协同识别因子为1.7。同时，该材料对四环素具有高选择性，印记因子为 7.6。此外，与表面修饰相比，通过分子印迹聚合物骨架中的交联有效地提高了适体的稳定性。对缓冲液、镁离子浓度、吸附时间等相关实验条件进行了优化。结果表明，该方法的检测限为 1.0 μg·L^{−1和0.005–0.5 mg L −1}的线性范围，以及一定的重现性和稳定性。此外，当应用于动物饲料样品的分析时，观察到基质干扰显着降低，回收率令人满意 (85.0–111.5%)，这强调了所建立方法的良好准确性和实用性。由于这些优点，所提出的方法代表了一种多功能且强大的工具，用于分离和检测复杂实际样品中的小分子化合物。