The analysis of low-abundant compounds with capillary zone electrophoresis–drift-tube ion mobility spectrometry–mass spectrometry (CZE–DTIMS–MS) is compromised due to the low injectable sample volumes in CZE and low duty cycle in DTIMS. Fritless packed in-line trap columns, using solid-phase extraction sorbent particles, have been used to increase injection volumes in CZE, but these columns are difficult to prepare and exhibit rapidly increasing back pressures. To provide smooth and complete filling of trap columns as well as to ensure higher and sustained flow rates though the columns, blends of cation and anion exchange particles with diatomite were used. The application of diatomite blends ensured the use of trap columns for at least 100 injections, with maximum injection volumes over 10 µl, which corresponds to an enrichment factor of more than 1000 over conventional injections in CZE–MS, enabling the detection of low nM concentrations of N-glycans with CZE–IMS–MS.

中文翻译：

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用于 CZE–IM–MS 大体积进样的带有硅藻土的在线样品捕获柱

由于 CZE 中的可注射样品量低和 DTIMS 中的低占空比，使用毛细管区带电泳-漂移管离子淌度谱-质谱 (CZE–DTIMS–MS) 分析低丰度化合物受到了影响。使用固相萃取吸附剂颗粒的无滤芯填充在线捕集柱已用于增加 CZE 中的进样量，但这些柱难以制备并且背压迅速增加。为了提供捕集柱的平滑和完全填充以及确保通过柱的更高和持续的流速，使用了阳离子和阴离子交换颗粒与硅藻土的混合物。硅藻土混合物的应用确保使用捕集柱进行至少 100 次进样，最大进样体积超过 10 µl，N-聚糖与 CZE–IMS–MS。