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A NIR Fluorescent Probe Benzopyrylium Perchlorate-based for Visual Sensing and Imaging of SO2 Derivatives in Living Cells
Journal of Fluorescence ( IF 2.6 ) Pub Date : 2022-11-04 , DOI: 10.1007/s10895-022-03050-w
Xiao Ye Luo 1 , Juan Xie 1 , Guang Lian Zhao 2 , Gui Yong Li 1 , Hang Da Qu 1 , Yu Zhu Yang 1
Affiliation  

Endogenous sulfur dioxide (SO2), as a gas signal molecule, has a certain physiological functions. Understanding the role of endogenous SO2 in human physiology and pathology is of great significance to the biological characteristics of SO2,which bring challenges to develop fluorescent probes with excellent performance. Herein, we rationally designed and constructed a novel near-infrared bioprobe benzaldehyde-benzopyrylium (BBp) by employing the nucleophilic addition benzopyrylium perchlorate fluorophore and benzaldehyde moiety by means of C = C/C = O group that serves as both fluorescence reporting unit. Probe BBp exhibit excellent sensing performance with fluorescent “On − Off”rapid response (100 s) and long-wavelength emission (670 nm). With the treatment of HSO3, the color of BBp solution obviously varies from purple to colorless, and the fluorescent color varies from red to colorless. By the fluorescence and colorimetric changes, probe BBp was capable of sensitive determination HSO3 with low limits of detection (LOD) of 0.43 μM, realizing visual quantitative monitoring SO2 derivative levels. Due to the low phototoxicity and good biocompatibility, it was successfully applied to monitor SO2 derivatives and fluorescence imaging in HepG2 and HeLa living cells. Hopefully, this work supplies a new strategy for designing NIR fluorescent probes for quantitative determination SO2 derivatives in biological samples.

Graphical Abstract



中文翻译:

基于高氯酸苯并吡喃的 NIR 荧光探针,用于活细胞中 SO2 衍生物的视觉传感和成像

内源性二氧化硫(SO 2 )作为一种气体信号分子,具有一定的生理功能。了解内源性SO 2在人体生理和病理中的作用对了解SO 2的生物学特性具有重要意义,这给开发性能优异的荧光探针带来了挑战。在此,我们合理设计并构建了一种新型近红外生物探针苯甲醛-苯并吡喃 (BBp),通过 C = C/C = O 基团的亲核加成苯并吡喃高氯酸盐荧光团和苯甲醛部分作为荧光报告单元。Probe BBp 具有出色的传感性能,具有荧光“开-关”快速响应(100 秒)和长波长发射(670 纳米)。随着HSO 3 -的处理,BBp溶液的颜色明显由紫色变为无色,荧光颜色由红色变为无色。通过荧光和比色变化,探针 BBp 能够灵敏地测定 HSO 3 -低检测限(LOD)0.43 μM,实现SO 2衍生物水平的可视化定量监测。由于低光毒性和良好的生物相容性,成功应用于监测HepG2和HeLa活细胞中的SO 2衍生物和荧光成像。希望这项工作为设计用于定量测定生物样品中SO 2衍生物的 NIR 荧光探针提供一种新策略。

图形概要

更新日期:2022-11-06
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