As one of the important metal ions, zinc ions (Zn²⁺) are widely involved in various physiological and pathological processes, and play fundamental roles in neurotransmission, cell metabolism and apoptosis. However, the convenient monitor of Zn²⁺ in environmental and biological samples remains challenging. In this study, a small molecule dicyanoisophorone-based schiff base incorporating with o-phenylenediamine was synthesized. It can rapidly combine with Zn²⁺ to emit significant near-infrared fluorescence (maximum emission wavelength: 660 nm), so it can be used as a probe to quantitatively detect Zn²⁺ in the range of 0–10 μM, with a detection limit as low as 4.8 nM, showing the probe has high sensitivity for Zn²⁺. And the probe has a fast response time to Zn²⁺ (less than 30 s) and a large Stoke-shift (179 nm). In addition, the high recovery rates in practical water samples, and the clear fluorescent images in living A549 cells were obtained, which are of great significance for the detection of Zn²⁺ in the environment and biosystem. Due to its simple operation, good selectivity and anti-interference ability, short detection time and high sensitivity, this probe has great application potential as a fast detection tool for Zn²⁺ in environmental water and biological samples.

^{锌离子(Zn 2+} )作为重要的金属离子之一，广泛参与多种生理和病理过程，在神经​​传递、细胞代谢和细胞凋亡等方面起着基础性作用。然而，环境和生物样品中 Zn ^{2+的便捷监测仍然具有挑战性。}在这项研究中，合成了一种与邻苯二胺结合的小分子二氰基异佛尔酮基席夫碱。^{能与Zn 2+}快速结合，发出显着的近红外荧光（最大发射波长：660 nm），因此可作为探针在0-10 μM范围内定量检测Zn ²⁺，具有检测限制低至 4.8 nM，表明探针对 Zn ^{2+具有高灵敏度}. 并且探头对Zn ²⁺响应时间快（小于30 s），斯托克位移大（179 nm）。此外，在实际水样中获得了高回收率，在活体A549细胞中获得了清晰的荧光图像，这对检测环境和生物系统中的Zn ^{2+具有重要意义。}该探针操作简单、选择性好、抗干扰能力强、检测时间短、灵敏度高，作为环境水体和生物样品中Zn ^{2+的快速检测工具具有巨大的应用潜力。}