Development of fluorescent imaging probes is an important topic of research for the early diagnosis of cancer. Based on the difference between the cellular environment of tumor cells and normal cells, several “smart” fluorescent probes have been developed. In this work, a glycopolymer functionalized Förster resonance energy transfer (FRET) based fluorescent sensor is developed, which can monitor the pH change in cellular system. One-pot sequential reversible addition-fragmentation chain transfer (RAFT)polymerization technique is employed to synthesize fluorescent active triblock glycopolymer that can undergo FRET change on the variation of pH. A FRET pair, fluorescein o-acrylate (FA) and 7-amino-4-methylcoumarin (AMC) is linked via a pH-responsive polymer poly [2-(diisopropylamino)ethyl methacrylate] (PDPAEMA), which can undergo reversible swelling/deswelling under acidic/neutral condition. The presence of glycopolymer segment provides stability, water solubility, and specificity toward cancer cells. The cellular FRET experiments on cancer cells (MDA MB 231) and normal cells (3T3 fibroblast cells) demonstrate that the material is capable of distinguishing cells as a function of pH change.

中文翻译：

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基于 Förster 共振能量转移的糖聚合物修饰的 pH 依赖性比例荧光探针用于检测癌细胞

开发荧光成像探针是癌症早期诊断的重要研究课题。基于肿瘤细胞与正常细胞的细胞环境差异，已经开发出多种“智能”荧光探针。在这项工作中，开发了一种基于糖聚合物功能化的 Förster 共振能量转移 (FRET) 荧光传感器，它可以监测细胞系统中的 pH 变化。采用一锅法顺序可逆加成-断裂链转移 (RAFT) 聚合技术合成了具有荧光活性的三嵌段糖聚合物，该三嵌段糖聚合物可随 pH 值的变化而发生 FRET 变化。FRET 对、荧光素 o-丙烯酸酯 (FA) 和 7-amino-4-methylcoumarin (AMC) 通过 pH 响应聚合物聚 [2-(diisopropylamino)ethyl methacrylate] (PDPAEMA) 连接，在酸性/中性条件下可发生可逆溶胀/消溶胀。糖聚合物片段的存在提供了稳定性、水溶性和对癌细胞的特异性。对癌细胞 (MDA MB 231) 和正常细胞（3T3 成纤维细胞）进行的细胞 FRET 实验表明，该材料能够根据 pH 变化区分细胞。