Hyssopus officinalis L. is one of the most important medicinal plants in traditional medicine used to treat seizures. In this study, we assessed the effects of H. officinalis hydroalcoholic extract against pentylenetetrazol (PTZ)-induced seizures in rat. The anti-seizure activity of the extract was assessed in three doses of 25, 50, and 100 mg/kg. Kindling was induced by intraperitoneal injection of PTZ (35 mg/kg) every 48 h, and H. officinalis extract was administered daily and behavioral tests performed. The possible involvement of GABA receptors in the extract activity was investigated using flumazenil. Tonic seizure threshold and mortality rate were measured following intraperitoneal injection of 60 mg/kg PTZ on the 14th day, following 14 days administration of H. officinalis hydroalcoholic extract. Blood and hippocampus samples were prepared to measure brain and serum antioxidant capacity, malondialdehyde (MDA), and nitric oxide (NO). Finally, the expression of GABA receptor gene in brain tissue was investigated. H. officinalis extract increased tonic seizure threshold and decreased mortality due to PTZ. Flumazenil, as a GABA receptor antagonist, reduced the tonic seizure threshold. Extract treatment significantly improved memory and learning, increased brain antioxidant capacity, decreased brain MDA and NO in kindled rats. It also increased GABA receptor gene expression in pre-treated groups compared to the negative control group. H. officinalis extract probably exerts potential antiepileptic effects through the GABAergic system. Also, H. officinalis extract has a supportive effect against hippocampal neuronal damage and improves memory and learning in kindled rats.

Hyssopus officinalis L. 是传统医学中用于治疗癫痫发作的最重要的药用植物之一。在这项研究中，我们评估了H. officinalis水醇提取物对戊四氮 (PTZ) 诱导的大鼠癫痫发作的影响。以 25、50 和 100 mg/kg 的三种剂量评估提取物的抗癫痫活性。通过每 48 小时腹膜内注射 PTZ (35 mg/kg) 和H诱导点燃。药用植物每天服用提取物并进行行为测试。使用氟马西尼研究了 GABA 受体可能参与提取物活性。在施用H. officinalis水醇提取物14 天后，在第 14 天腹膜内注射 60 mg/kg PTZ 后测量强直发作阈值和死亡率。制备血液和海马体样本以测量大脑和血清的抗氧化能力、丙二醛 (MDA) 和一氧化氮 (NO)。最后，研究了脑组织中GABA受体基因的表达。哈。药用植物提取物增加了强直发作阈值并降低了 PTZ 引起的死亡率。氟马西尼作为 GABA 受体拮抗剂，可降低强直性癫痫发作阈值。提取物处理显着改善了点燃大鼠的记忆和学习能力，增加了大脑的抗氧化能力，降低了大脑的 MDA 和 NO。与阴性对照组相比，它还增加了预处理组中的 GABA 受体基因表达。哈。药用提取物可能通过 GABAergic 系统发挥潜在的抗癫痫作用。此外，H。officinalis提取物对海马神经元损伤具有支持作用，并改善点燃大鼠的记忆和学习能力。