Richard S. Marshall, Lorenzo Frigerio and Lynne M. Roberts. Biochem. J. (2010) 427, 513–521. https://doi.org/10.1042/BJ20091878The authors of the original article “Disulfide formation in plant storage vacuoles permits assembly of a multimeric lectin” [Biochem. J. (2010) 427 (3): 513–521; https://doi.org/10.1042/BJ20091878] would like to correct errors in Figure 3B and Figure 5C. ‘In Figure 3B, the same set of three lanes (media) were re-used twice more. In Figure 5C, two lanes of an immunoblot containing smears were covered, in one instance with a copy of a neighbouring lane and in the other, with a lane from a portion of the film that was not actually shown.’ The corrected Figure 3B and Figure 5C are included in this Correction.The authors declare that the results and conclusions of the paper are not affected and remain supported by the data. ‘In the corrected Figure 3B we have removed the erroneously replicated control sections, leaving just the relevant set that show no proRCA emerged into the medium. It was already known from previous studies that there were never any RTA/RCA immunoprecipitable bands secreted from control plasmid (pDHA) cells and native proricin-transfected cells (JBC 1998 273:14194-9). This original publication revealed the same was also true for native proRCA-transfected cells. In Figure 5C the immunoblot demonstrated that mature seed proRCA (eluted from a column in fractions 64 – 70) was visualised in non-reducing PAGE as both ∼68kDa A-B and ∼136kDa tetrameric forms (in comparison with proricin (eluted in fractions 34-40) which was only seen as a ∼69kDa A-B form). This result remains valid, despite the smeared band in fraction 68.’ The authors apologise for these errors and any inconvenience they may have caused.

中文翻译：

---

植物储存液泡中二硫化物的形成允许组装多聚体凝集素

Richard S. Marshall、Lorenzo Frigerio 和 Lynne M. Roberts。生化。J. (2010) 427, 513–521。https://doi.org/10.1042/BJ20091878原始文章的作者“植物储存液泡中的二硫化物形成允许组装多聚体凝集素”[Biochem. J. (2010) 427 (3): 513–521；https://doi.org/10.1042/BJ20091878] 希望更正图 3B 和图 5C 中的错误。'在图 3B 中，同一组三个通道（媒体）被重复使用了两次。在图 5C 中，包含涂片的免疫印迹的两个泳道被覆盖，在一种情况下，有相邻泳道的副本，在另一种情况下，有一条来自实际未显示的胶片部分的泳道。更正后的图 3B 和图 5C 包含在此更正中。作者声明本文的结果和结论不受影响，并且仍然受到数据的支持。'在更正的图 3B 中，我们删除了错误复制的控制部分，只留下显示没有 proRCA 出现在培养基中的相关组。从之前的研究中已经知道，从对照质粒 (pDHA) 细胞和天然 proricin 转染的细胞中从未分泌过任何 RTA/RCA 免疫沉淀条带 (JBC 1998 273:14194-9)。该原始出版物揭示了对于天然 proRCA 转染的细胞也是如此。在图 5C 中，免疫印迹表明成熟的种子 proRCA（从 64-70 级分的柱中洗脱）在非还原 PAGE 中显示为 ~68kDa AB 和 ~136kDa 四聚体形式（与 proricin 相比（在级分 34-40 中洗脱） ）仅被视为~69kDa AB形式）。尽管分数 68 中存在拖尾带，但该结果仍然有效。