Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299†,Journal of Peptide Science

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Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299†
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2022-10-20 , DOI: 10.1002/psc.3458
Christoph J Albrecht _{1,

2} , Florian M Stumpf _{1,

2} , Lena Krüger _{1,

2} , Marie L Niedermeier _{2,

3} , Florian Stengel _{2,

3} , Andreas Marx _{1,

2}

Affiliation

Intracellular dinucleoside polyphosphates (Np_nNs) have been known for decades but the functional role remains enigmatic. Diadenosine triphosphate (Ap₃A) is one of the most prominent examples, and its intercellular concentration was shown to increase upon cellular stress. By employment of previously reported Ap₃A-based photoaffinity-labeling probes (PALPs) in chemical proteomics, we investigated the Ap₃A interactome in the human lung carcinoma cell line H1299. The cell line is deficient of the fragile histidine triade (Fhit) protein, a hydrolase of Ap₃A and tumor suppressor. Overall, the number of identified potential interaction partners was significantly lower than in the previously investigated HEK293T cell line. Gene ontology term analysis revealed that the identified proteins participate in similar pathways as for HEK293T, but the percentage of proteins involved in RNA-related processes is higher for H1299. The obtained results highlight similarities and differences of the Ap₃A interaction network in different cell lines and give further indications regarding the importance of the presence of Fhit.

中文翻译：

化学蛋白质组学揭示癌细胞系 H1299 中报警酮三磷酸二腺苷的相互作用因子†

细胞内二核苷多磷酸盐 (Np _n Ns) 已为人所知数十年，但其功能作用仍然是个谜。三磷酸二腺苷 (Ap ₃ A) 是最突出的例子之一，其细胞间浓度显示会随着细胞应激而增加。通过在化学蛋白质组学中使用先前报道的基于 Ap ₃ A 的光亲和标记探针 (PALP)，我们研究了人肺癌细胞系 H1299 中的Ap _{3 A 相互作用组。}该细胞系缺乏脆弱的组氨酸三联体 (Fhit) 蛋白，一种 Ap _{3的水解酶}A和肿瘤抑制因子。总体而言，已识别的潜在相互作用伙伴的数量明显低于先前研究的 HEK293T 细胞系。基因本体术语分析表明，所鉴定的蛋白质参与与 HEK293T 相似的途径，但 H1299 参与 RNA 相关过程的蛋白质百分比更高。_{获得的结果突出了 Ap 3} A 相互作用网络在不同细胞系中的异同，并进一步说明了 Fhit 存在的重要性。

更新日期：2022-10-20

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