High-performance blood plasma separation based on a Janus membrane technique and RBC agglutination reaction,Lab on a Chip

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High-performance blood plasma separation based on a Janus membrane technique and RBC agglutination reaction
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-10-18 , DOI: 10.1039/d2lc00508e
Bing Xu _{1,

2} , Juan Zhang ₁ , Deng Pan ₃ , Jincheng Ni ₄ , Kun Yin ₅ , Qilun Zhang ₆ , Yinlong Ding ₇ , Ang Li ₁ , Dong Wu ₇ , Zuojun Shen ₁

Affiliation

Separation of plasma which is full of various biomarkers is critical for clinical diagnosis. However, the point-of-care plasma separation often relies on microfluidic filtration membranes which are usually limited in purity, yield, hemolysis, extraction speed, hematocrit level, and protein recovery. Here, we have developed a high-performance plasma membrane separation technique based on a Janus membrane and red blood cell (RBC) agglutination reaction. The RBC agglutination reaction can form larger RBC aggregates to separate plasma from blood cells. Then, the Janus membrane, serving as a multipore microfilter to block large RBC aggregates, allows the plasma to flow from the hydrophobic side to its hydrophilic side spontaneously. As a result, the separation technique can extract highly-purified plasma (99.99%) from whole blood with an ultra-high plasma yield (∼80%) in ∼80 s. Additionally, the separation technique is independent of the hematocrit level and can avoid hemolysis.

中文翻译：

基于 Janus 膜技术和 RBC 凝集反应的高效血浆分离

分离富含各种生物标志物的血浆对于临床诊断至关重要。然而，床旁血浆分离通常依赖于微流体过滤膜，而微流体过滤膜通常在纯度、产量、溶血、提取速度、血细胞比容水平和蛋白质回收率方面受到限制。在这里，我们开发了一种基于 Janus 膜和红细胞 (RBC) 凝集反应的高性能质膜分离技术。RBC 凝集反应可以形成更大的 RBC 聚集体，从而将血浆与血细胞分离。然后，Janus 膜充当多孔微过滤器以阻挡大的 RBC 聚集体，使血浆自发地从疏水侧流向亲水侧。结果，分离技术可以提取高度纯化的血浆（99. 99% 来自全血，在 ~80 秒内具有超高血浆产量 (~80%)。此外，分离技术与血细胞比容水平无关，可以避免溶血。

更新日期：2022-10-18

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