The illegal addition of progesterone to cosmetics could cause serious adverse reactions and pose a serious threat to human health. In this work, a simple, fast and sensitive method was developed by combining molecularly imprinted solid-phase extraction and high-performance liquid chromatography (MISPE–HPLC) for the selective determination of progesterone in cosmetics. Chitosan-modified silica is used as the carrier to provide binding sites for the effective conjugation of the target. The obtained molecularly imprinted polymers exhibited excellent adsorption capacity (36.2 mg·g−1), good selectivity and fast mass transfer rate for progesterone. Meanwhile, the prepared MISPE column could eliminate the interference of co-existing substances. Combined MISPE with HPLC, a selective and effective method for detecting progesterone in different cosmetics was achieved. Under the optimum conditions, the established MISPE–HPLC method was successfully used for the detection of progesterone in real samples. The linear range of this method was 1 to 200 μg·mL−1 with a limit of detection of 0.016 μg·mL−1. Therefore, this method could be used for the selective and effective detection of progesterone in different cosmetic samples with complex substrates. We provided an alternative method for the detection of illegal additions in cosmetics.

中文翻译：

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基于分子印迹固相萃取和高效液相色谱法选择性分析化妆品样品中的黄体酮


化妆品中非法添加黄体酮可能会引起严重的不良反应，对人体健康造成严重威胁。在这项工作中，通过结合分子印迹固相萃取和高效液相色谱（MISPE-HPLC），开发了一种简单、快速、灵敏的方法，用于选择性测定化妆品中的黄体酮。壳聚糖改性二氧化硅用作载体，为靶标的有效缀合提供结合位点。所得分子印迹聚合物对黄体酮表现出优异的吸附容量（36.2 mg·g−1）、良好的选择性和快速的传质速率。同时，所制备的MISPE柱可以消除共存物质的干扰。将MISPE与HPLC相结合，建立了一种选择性、有效地检测不同化妆品中黄体酮的方法。在最佳条件下，所建立的MISPE-HPLC方法成功用于实际样品中黄体酮的检测。该方法的线性范围为1～200 μg·mL−1，检测限为0.016 μg·mL−1。因此，该方法可用于选择性、有效地检测具有复杂底物的不同化妆品样品中的孕酮。我们提供了一种检测化妆品中非法添加物的替代方法。