Rhizoctonia solani transcriptional activator interacts with rice WRKY53 and grassy tiller 1 to activate SWEET transporters for nutrition,Journal of Advanced Research

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Rhizoctonia solani transcriptional activator interacts with rice WRKY53 and grassy tiller 1 to activate SWEET transporters for nutrition
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-10-15 , DOI: 10.1016/j.jare.2022.10.001
Shuo Yang ₁ , Yuwen Fu ₁ , Yang Zhang ₁ , De Peng Yuan ₁ , Shuai Li ₁ , Vikranth Kumar ₂ , Qiong Mei ₁ , Yuan Hu Xuan ₁

Affiliation

Introduction

Rhizoctonia solani, the causative agent of the sheath blight disease (ShB), invades rice to obtain nutrients, especially sugars; however, the molecular mechanism via which R. solani hijacks sugars from rice remains unclear.

Objectives

In this study, rice-R. solani interaction model was used to explore whether pathogen effector proteins affect plant sugar absorption during infection.

Methods

Yeast one-hybrid assay was used to identify Activator of SWEET2a (AOS2) from R. solani. Localization and invertase secretion assays showed that nuclear localization and secreted function of AOS2. Hexose transport assays verified the hexose transporter activity of SWEET2a and SWEET3a. Yeast two-hybrid assays, Bimolecular fluorescence complementation (BiFC) and transactivation assay were conducted to verify the AOS2-WRKY53-Grassy tiller 1 (GT1) transcriptional complex and its activation of SWEET2a and SWEET3a. Genetic analysis is used to detect the response of GT1, WRKY53, SWEET2a, and SWEET3a to ShB infestation. Also, the soluble sugar contents were measured in the mutants and overexpression plants before and after the inoculation of R. solani.

Results

The present study found that R. solani protein AOS2 activates rice SWEET2a and localized in the nucleus of tobacco cells and secreted in yeast. AOS2 interacts with rice transcription factor WRKY53 and GT1 to form a complex that activates the hexose transporter gene SWEET2a and SWEET3a and negatively regulate rice resistance to ShB.

Conclusion

These data collectively suggest that AOS2 secreted by R. solani interacts with rice WRKY53 and GT1 to form a transcriptional complex that activates SWEETs to efflux sugars to apoplast; R. solani acquires more sugars and subsequently accelerates host invasion.

中文翻译：

立枯丝核菌转录激活因子与水稻WRKY53和草分蘖1相互作用，激活SWEET转运蛋白以获取营养

介绍

立枯丝核菌（ Rhizoctonia solani）是纹枯病（ShB）的病原体，它侵入水稻以获取营养，尤其是糖分；然而，立枯丝核菌从水稻中劫持糖的分子机制仍不清楚。

目标

在这项研究中，水稻-R。利用solani相互作用模型探讨病原体效应蛋白在感染过程中是否影响植物糖的吸收。

方法

采用酵母单杂交试验鉴定了立枯丝核菌中的SWEET2a ( AOS2 )激活剂。定位和转化酶分泌测定表明AOS2的核定位和分泌功能。己糖转运测定验证了 SWEET2a 和 SWEET3a 的己糖转运蛋白活性。进行酵母双杂交实验、双分子荧光互补（BiFC）和反式激活实验来验证AOS2-WRKY53-Grassy Tiller 1（GT1）转录复合物及其对SWEET2a和SWEET3a的激活。遗传分析用于检测GT1、WRKY53、SWEET2a和SWEET3a对 ShB 感染的反应。另外，在接种立枯丝核菌之前和之后测量了突变体和过表达植物中的可溶性糖含量。