Abstract

Chronic urticaria (CU) is a common disease characterized by the development of recurrent itchy blisters and/or angioedema lasting longer than 6 weeks. The evidence-based diagnosis of CU is described in the most recent urticaria guideline. Metabolomics has the potential to offer diagnostic biomarkers for the detection and prognosis of diseases and predict the efficacy and safety of pharmaceutical interventions. Determining the variation in metabolites found in the plasma of CU patients (n = 20) and 20 controls has therefore been the goal of this investigation. Samples were analyzed using liquid chromatography quadrupole time-of-flight mass spectrometry after applying acetonitrile precipitation. For the purpose of identifying and characterizing metabolites, the METLIN database was utilized. According to results, 21 metabolites were found to be significantly (VIP score > 0.7, p < .05 and fold analysis >1.5) altered. Differentiations between each group were successful via both OPLS-DA and ROC analysis. While plasma allantoate, homogentisate, indole acetate, proline, phenylalanine levels decreased in CU patients compared to healthy subjects, tryptophan, spermidine, phenyl pyruvate, oleic acid, lysine, valine, ornithine, histidine, glutamate, leucine, kynurenine, hypoxanthine, tyrosine, glucose, creatine and cortisol levels were significantly increased. Diagnosis of CU could be achieved by evaluating the metabolic profile of patients.

摘要

慢性荨麻疹 (CU) 是一种常见疾病，其特征是反复出现发痒的水疱和/或血管性水肿，持续时间超过 6 周。最新的荨麻疹指南描述了 CU 的循证诊断。代谢组学有潜力为疾病的检测和预后提供诊断生物标志物，并预测药物干预的有效性和安全性。因此，确定 CU 患者 ( n = 20) 和 20 名对照者血浆中代谢物的变化 是本次研究的目标。应用乙腈沉淀后，使用液相色谱四极杆飞行时间质谱分析样品。为了识别和表征代谢物，使用了 METLIN 数据库。根据结果​​，发现 21 种代谢物发生显着改变（VIP 评分 > 0.7，p  < .05 且倍数分析 > 1.5）。通过OPLS-DA 和 ROC 分析成功区分了每组。与健康受试者相比，CU患者血浆尿囊酸、黑黑酸、吲哚乙酸、脯氨酸、苯丙氨酸水平降低，色氨酸、亚精胺、丙酮酸苯酯、油酸、赖氨酸、缬氨酸、鸟氨酸、组氨酸、谷氨酸、亮氨酸、犬尿氨酸、次黄嘌呤、酪氨酸、葡萄糖、肌酸和皮质醇水平显着升高。CU 的诊断可以通过评估患者的代谢特征来实现。