A quenching electrochemiluminescence (ECL) immunosensor for neuron-specific enolase (NSE) detection was constructed by the resonance energy transfer between Ag@Ce₂Sn₂O₇ (energy donor) and Ag@Cu₂O (energy acceptor). The novel luminophore, Ag@Ce₂Sn₂O₇, was synthesized by complexing Ce₂Sn₂O₇ nanocubes with Ag concave-convex nanospheres. It exhibits a stronger ECL emission and a lower potential required for the emissions than pure Ce₂Sn₂O₇. The Ag@Cu₂O core-shell structure is composed of a dense Ag core inside and a loose Cu₂O shell that provide a large specific surface area and the protection for the independence and integrity of the Ag core. The UV-Vis absorption of Ag@Cu₂O overlaps the ECL emission of Ag@Ce₂Sn₂O₇, and thus it can act as a quencher to effectively quench the ECL signal of Ag@Ce₂Sn₂O₇. The great application potentials of the immunosensor in the NSE detection is demonstrated with the good linearity in the wide concentration range of 25 fg/mL - 100 ng/mL, and the low detection limit of 8.6 fg/mL. Our work has provided a reference for the immunosensor development of other biomarkers.

_{通过Ag@Ce 2} Sn ₂ O ₇（能量供体）和Ag@Cu ₂ O（能量受体）之间的共振能量转移构建了用于神经元特异性烯醇化酶（NSE）检测的淬灭电化学发光（ECL）免疫传感器。新型发光体Ag@Ce ₂ Sn ₂ O _{7是通过将Ce 2} Sn ₂ O ₇纳米立方体与Ag 凹凸纳米球络合而合成的。_{与纯Ce 2} Sn ₂ O ₇相比，它表现出更强的ECL发射和发射所需的更低电位。Ag@Cu ₂O核壳结构由内部致密的Ag核和松散的Cu ₂ O壳组成，提供了较大的比表面积，为Ag核的独立性和完整性提供了保护。_{Ag@Cu 2} O的紫外-可见吸收与Ag@Ce ₂ Sn ₂ O ₇的 ECL 发射重叠，因此它可以作为猝灭剂有效猝灭 Ag@Ce ₂ Sn ₂ O _{7的 ECL 信号}. 免疫传感器在 NSE 检测中的巨大应用潜力体现在 25 fg/mL - 100 ng/mL 的宽浓度范围内具有良好的线性，以及 8.6 fg/mL 的低检测限。我们的工作为其他生物标志物的免疫传感器开发提供了参考。