“Shutter” Effects Enhance Protein Diffusion in Dynamic and Rigid Molecular Networks,Journal of the American Chemical Society

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“Shutter” Effects Enhance Protein Diffusion in Dynamic and Rigid Molecular Networks
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2022-10-05 , DOI: 10.1021/jacs.2c07830
Xiaobin Dai ₁ , Zhichao Zhu ₂ , Yujie Li ₂ , Bo Yang ₂ , Jiang-Fei Xu ₂ , Yuanchen Dong ₃ , Xin Zhou ₄ , Li-Tang Yan ₁ , Dongsheng Liu ₂

Affiliation

Hydrogels have been widely applied to understand the fundamental functions and mechanism of a natural extracellular matrix (ECM). However, revealing the high permeability of ECM through synthetic hydrogels is still challenged by constructing analogue networks with rigid and dynamic properties. Here, in this study, taking advantage of the rigidity and dynamic binding of DNA building blocks, we have designed a model hydrogel system with structural similarity to ECM, leading to enhanced diffusion for proteins compared with a synthetic polyacrylamide (PAAm) hydrogel. The molecular diffusion behaviors in such a rigid and dynamic network have been investigated both in experiments and simulations, and the dependence of diffusion coefficients with respect to molecular size exhibits a unique transition from a power law to an exponential function. A “shutter” model based on the rigid and dynamic molecular network has been proposed, which has successfully revealed how the rigidity and dynamic bond exchange determine the diffusion mechanism, potentially providing a novel perspective to understand the possible mechanism of enhanced diffusion behaviors in ECM.

中文翻译：

“快门”效应增强了动态和刚性分子网络中的蛋白质扩散

水凝胶已被广泛应用于了解天然细胞外基质 (ECM) 的基本功能和机制。然而，通过构建具有刚性和动态特性的模拟网络，通过合成水凝胶揭示 ECM 的高渗透性仍然面临挑战。在这项研究中，我们利用 DNA 构建块的刚性和动态结合，设计了一种与 ECM 结构相似的模型水凝胶系统，与合成聚丙烯酰胺 (PAAm) 水凝胶相比，可增强蛋白质的扩散。已经在实验和模拟中研究了这种刚性和动态网络中的分子扩散行为，扩散系数对分子大小的依赖性表现出从幂律到指数函数的独特转变。

更新日期：2022-10-05

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