During this study, a simple and easy-to-prepare electrophoretic method was developed for the enantioseparation of amphetamine and cathinone derivatives. Different types of β-cyclodextrin and cyclofructan-based chiral selectors (CSs), both native and derivatized, were utilized, and the most effective ones, in terms of resolution and analysis time, were identified. In addition, several electrophoretic parameters, such as background electrolyte concentration and pH, and CS concentration, were examined to optimize the separation conditions. Under the optimal electrophoretic conditions, 10 psychoactive substances were enantiomerically separated using 1 mM sulfated cyclofructan-6 (SCF-6) for the amphetamine derivatives and 1 mM sulfated cyclofructan-7 (SCF-7) for the cathinone derivatives dissolved in an aqueous solution of 20-mM monobasic sodium phosphate at pH 2.5, a temperature of 25°C, and an applied voltage of 25 kV. In addition, the method was validated by estimating the intra- and interday precision.

中文翻译：

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基于环糊精和环果聚糖的手性选择剂在毛细管电泳中对精神活性物质对映分离的评价

在这项研究中，开发了一种简单且易于制备的电泳方法，用于苯丙胺和卡西酮衍生物的对映体分离。使用了不同类型的 β-环糊精和基于环果聚糖的手性选择器 (CS)，包括天然的和衍生化的，并确定了在分辨率和分析时间方面最有效的选择器。此外，还检查了几个电泳参数，例如背景电解质浓度和 pH 值以及 CS 浓度，以优化分离条件。在最佳电泳条件下，10 种精神活性物质对苯丙胺衍生物使用 1 mM 硫酸化环果聚糖 - 6 (SCF-6) 和卡西酮衍生物使用 1 mM 硫酸化环果聚糖 - 7 (SCF-7) 溶解在 20-mM 磷酸二氢钠水溶液中进行对映体分离在 pH 值 2.5、温度 25°C 和施加电压 25 kV 的条件下。此外，通过估计日内和日间精密度来验证该方法。