Reasonable and efficient removal of mixed pollutants (nitrate and heavy metals) in industrial wastewater under heavy metal pollution has attracted more attention in recent years. The target strain Aquabacterium sp. XL4 was immobilized with different iron ore wastes (IOW) using polyvinyl alcohol (PVA) to construct four immobilized bioreactors. The results showed that when the ratio of C/N was 1.5 and the hydraulic retention time (HRT) was 8.0h, the denitrification performance of the bioreactor was the best, and the maximum denitrification efficiency of the bioreactor with sponge iron (SI) as the iron source was 97.19% (2.42mg L^–1 h^–1). Furthermore, by adjusting the concentration of Cu²⁺ and Pb²⁺, the stress behavior of the bioreactor to heavy metals under the influence of each IOW was investigated. The bioreactor has stronger tolerance and removal efficiency to Pb²⁺ and Cu²⁺ in the presence of pellets ore (PO) and refined iron ore (RO), respectively. Moreover, the high–throughput data showed that Aquabacterium accounted for a high proportion in the immobilized bioreactor, and the prediction of functional genes based on the KEGG database showed that the addition of IOW was closely related to the acceleration of nitrate transformation and the inflow and outflow of iron in cells.

近年来，重金属污染下工业废水中混合污染物（硝酸盐和重金属）的合理高效去除越来越受到关注。目标菌株Aquabacterium sp。XL4 使用聚乙烯醇 (PVA) 与不同的铁矿石废料 (IOW) 固定化，以构建四个固定化生物反应器。结果表明,当C/N比为1.5、水力停留时间(HRT)为8.0h时,生物反应器的反硝化性能最好,海绵铁(SI)生物反应器的最大反硝化效率为铁源为 97.19% (2.42mg L ^–1 h ^–1 )。此外，通过调节Cu ²⁺和Pb ^2+的浓度, 研究了生物反应器在每种 IOW 影响下对重金属的应力行为。该生物反应器在球团矿(PO)和精炼铁矿(RO)分别存在的情况下对Pb ²⁺和Cu ²⁺具有更强的耐受性和去除效率。此外，高通量数据显示，水生菌在固定化生物反应器中占比较高，基于KEGG数据库的功能基因预测表明，IOW的加入与硝酸盐转化的加速和流入和流出密切相关。细胞中铁的流出。