Scorpion peptides have good therapeutic effect on chronic ulcer of diabetic foot, but the related pharmacological mechanism has remained unclear. The different proteins and bacteria present in ulcer exudates from chronic diabetic foot patients, treated with scorpion antimicrobial peptide at different stages, were analyzed using isobaric tags for quantification-labeled proteomics and bacteriological methods. According to the mass spectrometry data, a total of 1865 proteins were identified qualitatively, and the number of the different proteins was 130 (mid/early), 401 (late/early), and 310 (mid, late/early). In addition, functional annotation, cluster analysis of effects and the analysis of signal pathway, transcription regulation, and protein-protein interaction network were carried out. The results showed that the biochemical changes of wound microenvironment during the treatment involved activated biological functions such as protein synthesis, cell proliferation, differentiation, migration, movement, and survival. Inhibited biological functions such as cell death, inflammatory response, immune diseases, and bacterial growth were also involved. Bacteriological analysis showed that Burkholderia cepacia was the main bacteria in the early and middle stage of ulcer exudate and Staphylococcus epidermidis in the late stage. This study provides basic data for further elucidation of the molecular mechanism of diabetic foot.

中文翻译：

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全蝎抗菌肽治疗糖尿病足慢性溃疡渗出液的蛋白质组学分析

蝎肽对糖尿病足慢性溃疡有良好的治疗作用，但其相关药理机制尚不清楚。在不同阶段用蝎子抗菌肽治疗的慢性糖尿病足患者的溃疡渗出物中存在的不同蛋白质和细菌，使用等压标签进行定量标记的蛋白质组学和细菌学方法进行分析。根据质谱数据，共定性鉴定了1865个蛋白质，不同蛋白质的数量分别为130（中/早）、401（晚/早）和310（中、晚/早）。此外，还进行了功能注释、效应聚类分析和信号通路分析、转录调控和蛋白质-蛋白质相互作用网络分析。结果表明，治疗过程中伤口微环境的生化变化涉及激活的生物学功能，如蛋白质合成、细胞增殖、分化、迁移、运动和存活。还涉及受抑制的生物学功能，例如细胞死亡、炎症反应、免疫疾病和细菌生长。细菌学分析表明洋葱伯克霍尔德菌是溃疡渗出液早期和中期的主要细菌，晚期是表皮葡萄球菌。本研究为进一步阐明糖尿病足的分子机制提供了基础数据。