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Phage Predation Promotes Filamentous Bacterium Piscinibacter Colonization and Improves Structural and Hydraulic Stability of Microbial Aggregates
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-09-29 , DOI: 10.1021/acs.est.2c04745 Zhuodong Yu 1 , Juhong Chen 2 , Yixiao Tan 1 , Yun Shen 3 , Liang Zhu 1 , Pingfeng Yu 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-09-29 , DOI: 10.1021/acs.est.2c04745 Zhuodong Yu 1 , Juhong Chen 2 , Yixiao Tan 1 , Yun Shen 3 , Liang Zhu 1 , Pingfeng Yu 1
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Although bacteria–phage interactions have broad environmental applications and ecological implications, the influence of phage predation on bacterial aggregation and structural stability remains largely unexplored. Herein, we demonstrate that inefficient lytic phage predation can promote host filamentous bacterium Piscinibacter colonization onto non-host Thauera aggregates, improving the structural and hydraulic stability of the dual-species aggregates. Specifically, phage predation at 103–104 PFU/mL (i.e., multiplication of infection at 0.01–0.1) promoted initial Piscinibacter colonization by 10–15 folds and resulted in 29–31% higher abundance of Piscinibacter in the stabilized aggregates than that in the control aggregates without phage predation. Transcriptomic analysis revealed upregulated genes related to quorum sensing (by 15–92 folds) and polysaccharide secretion (by 10–90 folds) within the treated aggregates, which was consistent with 120–172% higher content of polysaccharides for the treated dual-species aggregates. Confocal laser scanning microscopic images further confirmed the increase of filamentous bacteria and polysaccharides (both with wider distribution) within the dual-species aggregates. Accordlingly, the aggregates’ structural strength (via atomic force microscopes) and shear resistance (via hydraulic stress tests) increased by 77 and 42%, respectively, relative to the control group. In the long-term experiments, the enhanced hydraulic stability of the treated aggregates could facilitate dwelling bacteria propagation in flow-through conditions. Overall, our study demonstrates that phage predation can promote bacterial aggregation and enhance aggregate structural stability, revealing the beneficial role of lytic phage predation on bacterial symbiosis and environmental adaptivity.
中文翻译：
噬菌体捕食促进丝状细菌 Piscinibacter 定植并提高微生物聚集体的结构和水力稳定性
尽管细菌-噬菌体相互作用具有广泛的环境应用和生态意义，但噬菌体捕食对细菌聚集和结构稳定性的影响在很大程度上仍未得到探索。在此，我们证明低效的裂解噬菌体捕食可以促进宿主丝状细菌Piscinibacter定植到非宿主Thauera聚集体上，提高双物种聚集体的结构和水力稳定性。具体而言，10 3 –10 4 PFU/mL的噬菌体捕食（即感染倍增 0.01–0.1）将Piscinibacter的初始定殖提高了 10–15 倍，并导致Piscinibacter的丰度提高了 29–31%在稳定的聚集体中比在没有噬菌体捕食的对照聚集体中。转录组学分析显示，处理过的聚集体中与群体感应（15-92 倍）和多糖分泌（10-90 倍）相关的基因上调，这与处理过的双物种聚集体的多糖含量高出 120-172% 一致. 共聚焦激光扫描显微图像进一步证实了双物种聚集体中丝状细菌和多糖（均具有更广泛的分布）的增加。因此，相对于对照组，聚集体的结构强度（通过原子力显微镜）和抗剪强度（通过水压试验）分别增加了 77% 和 42%。在长期的实验中，经处理的聚集体增强的水力稳定性可以促进细菌在流通条件下的繁殖。总的来说，我们的研究表明噬菌体捕食可以促进细菌聚集并增强聚集体结构稳定性，揭示了裂解性噬菌体捕食对细菌共生和环境适应性的有益作用。
更新日期：2022-09-29
中文翻译：
噬菌体捕食促进丝状细菌 Piscinibacter 定植并提高微生物聚集体的结构和水力稳定性
尽管细菌-噬菌体相互作用具有广泛的环境应用和生态意义，但噬菌体捕食对细菌聚集和结构稳定性的影响在很大程度上仍未得到探索。在此，我们证明低效的裂解噬菌体捕食可以促进宿主丝状细菌Piscinibacter定植到非宿主Thauera聚集体上，提高双物种聚集体的结构和水力稳定性。具体而言，10 3 –10 4 PFU/mL的噬菌体捕食（即感染倍增 0.01–0.1）将Piscinibacter的初始定殖提高了 10–15 倍，并导致Piscinibacter的丰度提高了 29–31%在稳定的聚集体中比在没有噬菌体捕食的对照聚集体中。转录组学分析显示，处理过的聚集体中与群体感应（15-92 倍）和多糖分泌（10-90 倍）相关的基因上调，这与处理过的双物种聚集体的多糖含量高出 120-172% 一致. 共聚焦激光扫描显微图像进一步证实了双物种聚集体中丝状细菌和多糖（均具有更广泛的分布）的增加。因此，相对于对照组，聚集体的结构强度（通过原子力显微镜）和抗剪强度（通过水压试验）分别增加了 77% 和 42%。在长期的实验中，经处理的聚集体增强的水力稳定性可以促进细菌在流通条件下的繁殖。总的来说，我们的研究表明噬菌体捕食可以促进细菌聚集并增强聚集体结构稳定性，揭示了裂解性噬菌体捕食对细菌共生和环境适应性的有益作用。
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