Micromelalopha troglodyta (Graeser) has been one of the most serious pests on poplars in China. We used Illumina HiSeq 2000 sequencing to construct an antennal transcriptome and identify olfactory-related genes. In total, 142 transcripts were identified, including 74 odorant receptors (ORs), 32 odorant-binding proteins (OBPs), 13 chemosensory proteins (CSPs), 20 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). The genetic relationships were obtained by the phylogenetic tree, and the tissue-specific expression of important olfactory-related genes was determined by quantitative real-time PCR (qRT-PCR). The results showed that most of these genes are abundantly expressed in the antennae and head. In most insects, olfaction plays a key role in foraging, host localization, and searching for mates. Our research lays the foundation for future research on the molecular mechanism of the olfactory system in M. troglodyta. In addition, this study provides a theoretical basis for exploring the relationship between M. troglodyta and their host plants, and for the biological control of M. troglodyta using olfactory receptor as targets.

中文翻译：

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Micromelalopha troglodyta (Lepidoptera: Notodontidae) 的触角转录组分析和嗅觉相关基因组织特异性表达的鉴定。

穴居小斑蝽（Graeser）一直是我国杨树危害最严重的害虫之一。我们使用 Illumina HiSeq 2000 测序构建触角转录组并鉴定嗅觉相关基因。总共鉴定了 142 个转录本，包括 74 个气味受体 (OR)、32 个气味结合蛋白 (OBP)、13 个化学感觉蛋白 (CSP)、20 个离子型受体 (IR) 和 3 个感觉神经元膜蛋白 (SNMP)。通过系统发育树获得遗传关系，并通过定量实时PCR（qRT-PCR）确定重要嗅觉相关基因的组织特异性表达。结果表明，这些基因中的大多数在触角和头部中大量表达。在大多数昆虫中，嗅觉在觅食、宿主定位和寻找配偶中起着关键作用。我们的研究为今后深入研究穴居蛙嗅觉系统的分子机制奠定了基础。此外，本研究为探索穴居地蛉与其寄主植物的关系，以及以嗅觉受体为靶点的穴居地蚌生物防治提供了理论依据。