Purpose: This study aims to identify key genes in slow transit constipation (STC). We also sought to explore the potential link between STC and colorectal cancer.
Patients and Methods: mRNA expression profiles were obtained by RNA sequencing, and differentially expressed genes were identified. Functional enrichment analysis and a protein–protein interaction (PPI) network was explored, and differentially expressed genes common to STC and colorectal cancer were examined. Analysis of the effect of constipation and colorectal cancer common genes on the overall survival of colorectal cancer patients based on GEPIA database.
Results: Functional enrichment showed that significantly different genes are related to lymphocyte chemotaxis, positive regulation of inflammatory response, cellular response to tumor necrosis factor, extracellular region, extracellular space and chemokine activity. The hub gene for STC was found in the PPI network. In addition, AQP8 and CFD were common differential genes for STC and colorectal cancer. AQP8 affects overall survival in patients with colorectal cancer.
Conclusion: Our findings will contribute to understanding the pathology of STC at the molecular level, with the first discovery that AQP8 may be a hub gene in the transition from STC to colorectal cancer.

Keywords: slow transit constipation, RNA sequencing, hub genes, colorectal cancer, AQP8


中文翻译：

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基于RNA测序的慢转运型便秘关键基因分析

目的：本研究旨在确定慢传输型便秘（STC）的关键基因。我们还试图探索 STC 与结直肠癌之间的潜在联系。
患者和方法：通过RNA测序获得mRNA表达谱，并鉴定差异表达基因。探索了功能富集分析和蛋白质-蛋白质相互作用（PPI）网络，并检查了STC和结直肠癌共有的差异表达基因。基于GEPIA数据库的便秘与结直肠癌共同基因对结直肠癌患者总生存期的影响分析
结果：功能富集显示，显着不同的基因与淋巴细胞趋化性、炎症反应的正调节、细胞对肿瘤坏死因子的反应、细胞外区域、细胞外空间和趋化因子活性有关。在 PPI 网络中发现了 STC 的中心基因。此外，AQP8和CFD是STC和结直肠癌的常见差异基因。AQP8 影响结直肠癌患者的总生存期。
结论：我们的研究结果将有助于在分子水平上理解STC的病理学，首次发现AQP8可能是STC向结直肠癌转变的中枢基因。

关键词：慢传输型便秘，RNA测序，枢纽基因，结直肠癌，AQP8