Engineered CRISPR prime editors with compact, untethered reverse transcriptases,Nature Biotechnology

当前位置： X-MOL 学术 › Nat. Biotechnol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Engineered CRISPR prime editors with compact, untethered reverse transcriptases
Nature Biotechnology ( IF 33.1 ) Pub Date : 2022-09-26 , DOI: 10.1038/s41587-022-01473-1
Julian Grünewald _{1,

2,

3,

4,

5,

6,

7} , Bret R Miller _{1,

2} , Regan N Szalay _{1,

2} , Peter K Cabeceiras _{1,

2} , Christopher J Woodilla _{1,

2} , Eliza Jane B Holtz _{1,

2} , Karl Petri _{1,

2,

3} , J Keith Joung _{1,

2,

3}

Affiliation

The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.

中文翻译：

具有紧凑、不受束缚的逆转录酶的工程化 CRISPR prime 编辑器

CRISPR 主编辑器 PE2 由C 端与莫洛尼鼠白血病病毒逆转录酶 (MMLV-RT) 融合的化脓性链球菌Cas9 切口酶 (nSpCas9) 组成。在这里，我们展示了分离的 nSpCas9 和 MMLV-RT 蛋白在人类细胞中与完整的 PE2 一样有效地发挥作用。我们使用这个 Split-PE 系统来快速识别和设计更紧凑的 Prime 编辑器架构，这也拓宽了用于 Prime 编辑的 RT 类型。

更新日期：2022-09-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11