High demands of medical and husbandry sectors led to a massive annual production of 100,000 tonnes of various antibiotics on a global scale. Considering that an access to sublethal doses of these pharmaceuticals causes subsequent increase in resistance of virulent human bacterial pathogens, there is a substantial need for development of novel methods and techniques for degradation of antibiotics from aqueous solutions. Here, high-throughput continuous flow plasma pencil and plasma brush methodologies were developed and verified for their applicability for removal of antibiotics from solutions, as estimated by high-performance liquid chromatography coupled with diode-array detection (HPLC-DAD) or ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The removal efficiency of drugs treated by the plasma pencil was in the range from 3 to 24%, and was corresponded to with 1.39% to 10.76% losses in the antibacterial properties of the plasma-treated solutions. However, application of a unique plasma brush increased the antibiotics degradation rates significantly, now falling in the range from 29 to 67% that led to 12.06–81.59% decrease in the antimicrobial properties of the plasma-exposed antibiotics solutions. The deviations in the effectiveness of the plasma pencil and the plasma brush methods were assigned with the types and amounts of generated species. Various intermediate degradation products were also detected in the post-plasma solutions, which derived from the oxygen attack to nucleophilic sides of the antibiotic molecules. We assume that such novel high-throughput, continuous flow methodological setups may find application in hospitals as an end-of-pipe technology for routine elimination of pollutants before reaching municipal sewage treatment plants.

医疗和畜牧业的高需求导致全球每年大量生产10万吨各种抗生素。考虑到获得亚致死剂量的这些药物会导致随后增加对有毒人类细菌病原体的抵抗力，因此非常需要开发从水溶液中降解抗生素的新方法和技术。在这里，通过高效液相色谱结合二极管阵列检测 (HPLC-DAD) 或超高效液相色谱法，开发并验证了高通量连续流动等离子笔和等离子刷方法从溶液中去除抗生素的适用性与串联质谱 (UPLC-MS/MS) 相结合。等离子笔处理的药物去除效率在 3% 到 24% 之间，与等离子处理溶液的抗菌性能损失相对应的为 1.39% 到 10.76%。然而，独特的等离子刷的应用显着提高了抗生素降解率，现在下降在 29% 至 67% 的范围内，导致暴露于血浆的抗生素溶液的抗菌性能下降 12.06% 至 81.59%。等离子笔和等离子刷方法的有效性偏差与生成物质的类型和数量有关。在血浆后溶液中还检测到各种中间降解产物，这些中间降解产物源自氧对抗生素分子亲核侧的攻击。我们假设这种新颖的高通量，