Effect of species, concentration and volume of local anesthetics on intervertebral disk degeneration in rats with discoblock,European Spine Journal

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Effect of species, concentration and volume of local anesthetics on intervertebral disk degeneration in rats with discoblock
European Spine Journal ( IF 2.6 ) Pub Date : 2022-09-24 , DOI: 10.1007/s00586-022-07398-2
Weiheng Wang ₁ , Bing Xiao ₁ , Lei Yu ₂ , Haotian Wang ₁ , Junqiang Qi ₁ , Yanhai Xi ₁ , Guoying Deng ₃ , Xin Gu ₄ , Guohua Xu ₁

Affiliation

Purpose

Discoblock is effective in relieving discogenic low back pain, but it can also cause intervertebral disk degeneration (IDD). The effect of species, concentration and volume of local anesthetics on IDD with discoblock have not been reported. The purpose was to study the effect of species, concentration and volume of local anesthetics on IDD in rats undergoing discoblock.

Methods

The effects of local anesthetics on nucleus pulposus cell (NPC) viability in vitro were studied. NPCs were exposed to lidocaine, bupivacaine and ropivacaine at different concentrations. NPC viability was measured. The least cytotoxic local anesthetic was used in vivo. The concentration and volume of local anesthetics on IDD in rat with discoblocks were tested in vivo. Detection indicators included X-ray, MRI, water content of the disk and histological changes.

Results

The toxicity of local anesthetics to NPCs was dose and time dependent, and the cytotoxicity of different local anesthetics was different. Among the three local anesthetics, ropivacaine was the least toxic to NPCs. The effect of ropivacaine concentration on IDD was not significant, as detected by X-ray, MRI, disk water content and histology (P < 0.05). The volume of ropivacaine has a significant effect on IDD, as supported by X-ray, MRI, disk water content and histology (P < 0.05). Acupuncture itself significantly increased IDD, as detected by MRI, disk water content and histology (P < 0.05).

Conclusion

Ropivacaine should be selected for its low cytotoxicity. A lower volume and slow injection speed should be used to reduce IDD during discoblock.

中文翻译：

局麻药种类、浓度和体积对discoblock大鼠椎间盘退变的影响

目的

Discoblock 可有效缓解椎间盘源性腰痛，但它也会引起椎间盘退变 (IDD)。局部麻醉药的种类、浓度和体积对 discoblock 引起的 IDD 的影响尚未见报道。目的是研究局部麻醉剂的种类、浓度和体积对接受 discoblock 的大鼠 IDD 的影响。

方法

研究了局麻药对体外髓核细胞 (NPC) 活力的影响。NPC 暴露于不同浓度的利多卡因、布比卡因和罗哌卡因。测量了 NPC 的生存能力。在体内使用细胞毒性最小的局部麻醉剂。在体内测试了局部麻醉剂对 discoblocks 大鼠 IDD 的浓度和体积。检测指标包括X线、MRI、椎间盘含水量和组织学变化。

结果

局麻药对鼻咽癌的毒性呈剂量和时间依赖性，不同局麻药的细胞毒性不同。在三种局麻药中，罗哌卡因对鼻咽癌的毒性最小。罗哌卡因浓度对 IDD 的影响不显着，如 X 线、MRI、纸片含水量和组织学检测（P < 0.05）。罗哌卡因用量对 IDD 有显着影响，X 线、MRI、纸片含水量和组织学均支持（P < 0.05）。针灸本身显着增加了 IDD，如通过 MRI、磁盘含水量和组织学检测到的（P < 0.05）。