Extraction methods depend mainly on the chemical nature of the extracted molecule. For these reasons, the selection of the extraction medium is a vital part of obtaining these molecules. The extraction of antimicrobial peptides (AMPs) from extremophile plants is important because of its potential pharmaceutical applications. This work focused on the evaluation of several solvents for the extraction of AMPs from the following two extremophile plants: Astragalus armatus and Anthyllis sericea from southern Tunisia. In order to identify the most efficient solvents and extraction solutions, we used sulfuric acid, dichloromethane, phosphate buffer, acetic acid and sodium acetate, and we tested them on leaves and roots of both the studied plants. The extracts obtained using sulfuric acid, dichloromethane and phosphate buffer extraction did not show any antimicrobial activity, whereas the acetic acid and sodium acetate extracts led to growth inhibition of some of the tested bacterial strains. The extracts of leaves and roots of An. sericea and As. armatus obtained by acetic acid and sodium acetate were proven to be active against Gram-positive bacteria and Gram-negative bacteria. Therefore, the most appropriate solvents to use for antimicrobial peptide extraction from both plants are acetic acid and sodium acetate.

中文翻译：

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从突尼斯沙漠采集的极端植物根叶抗菌肽的优化化学提取方法

提取方法主要取决于提取分子的化学性质。由于这些原因，提取介质的选择是获得这些分子的重要部分。从极端微生物植物中提取抗菌肽 (AMP) 非常重要，因为它具有潜在的药物应用价值。这项工作的重点是评估用于从以下两种极端植物中提取 AMP 的几种溶剂：Astragalus armatus和Anthyllis sericea来自突尼斯南部。为了确定最有效的溶剂和提取溶液，我们使用了硫酸、二氯甲烷、磷酸盐缓冲液、乙酸和乙酸钠，并在两种研究植物的叶和根上进行了测试。使用硫酸、二氯甲烷和磷酸盐缓冲液提取获得的提取物没有显示出任何抗微生物活性，而乙酸和乙酸钠提取物导致一些测试菌株的生长抑制。安的叶和根的提取物。丝绸和作为。阿玛图斯由醋酸和醋酸钠获得的醋酸和醋酸钠被证明对革兰氏阳性菌和革兰氏阴性菌有活性。因此，用于从两种植物中提取抗菌肽的最合适的溶剂是乙酸和乙酸钠。