Accurate identification and effective removal of unwanted variation is essential to derive meaningful biological results from large and complex RNA-seq studies. Technical replicates together with negative and positive control genes are key tools for carrying out this task. We show how to proceed when technical replicates are unavailable.

中文翻译：

---

针对文库大小、肿瘤纯度和批次效应对癌症 RNA-seq 数据进行标准化

准确识别和有效去除不需要的变异对于从大型复杂的 RNA-seq 研究中获得有意义的生物学结果至关重要。技术复制以及阴性和阳性对照基因是执行此任务的关键工具。我们展示了在技术复制不可用时如何进行。